Used Thermo Fisher High Resolution Mass Spectrometer QE QE HF

Used Thermo Fisher High Resolution Mass Spectrometer QE QE HFThe ultra-high electrostatic field orbit trap Fourier transform bench mass spectrometry combines high-performance quadrupole rods that provide parent ion selection and ultra-high electrostatic field orbit traps with ultra-high resolution and high-quality accuracy. With faster detection speed and higher resolution, it has better performance in applications such as peptides, proteins, and small molecules.

Thermo Fisher has added an ultra-high field Orbitrap mass analyzer to the validated Q Exactive Plus LC-MS system. This system is designed for faster identification, characterization, quantification, and confirmation in a highly reliable analysis; In order to significantly improve analysis efficiency, the maximum scanning speed has been increased by 50%. The Q Exactive HF system can complete dozens of complete MSMS scans in 1 second.

The Thermo high-resolution mass spectrometry qualitative and quantitative system is mainly used for protein high-resolution, high-precision identification and quantitative research in proteomics research. It is suitable for efficient separation of complex protein and peptide mixtures and for high-sensitivity, high accuracy and high-throughput analysis. In addition, it can also achieve multi-stage tandem mass spectrometry function to facilitate mass spectrometry based structural analysis and protein relative quantification.

Technical specifications: Scanning speed up to 18 Hz; resolution up to 240000FWHM.

Application scope: Proteomic analysis. The Q Exactive HF mass spectrometer supports multiple quantitative methods, including Selective Ion Monitoring (SIM), Parallel Reaction Monitoring (PRM), and Data Independence Acquisition (DIA)

What isUsed Thermo Fisher High Resolution Mass Spectrometer QE QE HF

The Q Exactive mass spectrometer is a combination of quadrupole and high-precision Orbitrap mass spectrometry technologyA high-performance mass spectrometer. This instrument can provide high-precision, high-resolution, and high-sensitivity mass spectrometry data, and is currently the mainstream equipment for proteomics analysis.

How to perform proteomic analysis with Q Exactive mass spectrometer

Pre processing of protein samples

The first step in proteomic analysis is sample processing, which typically includes protein extraction, purification, digestion into peptide segments, and peptide washing. The purpose of this stage is to transform complex protein samples into peptide mixtures that can be directly analyzed by mass spectrometry.

Liquid chromatography separation of peptide segments

The preprocessed peptide samples will be sent to a liquid chromatography system for separation. This step can reduce sample complexity and improve the sensitivity and accuracy of mass spectrometry analysis.

Mass spectrometry detection and data acquisition

The separated peptide will pass through the electric spray ion source(ESI) Enter the Q Exactive mass spectrometer for detection. The quadrupole of the mass spectrometer can provide efficient peptide ionization and secondary mass spectrometry analysis, while the Orbitrap detector can provide high-resolution and high-precision mass spectrometry data.

Data processing and protein identification

The data processing software equipped with the Q Exactive mass spectrometer can automatically identify peptides and proteins, including database search, protein quantification, post-translational modification analysis, and other functions.

Advantages of Q Exactive Mass Spectrometer in Proteomics

The Q Exactive mass spectrometer provides high-resolution and high-precision mass spectrometry data, making protein identification and quantification more accurate. In addition, its data processing capabilities have improved the efficiency and depth of proteomic analysis. In addition, the Q Exactive mass spectrometer can adapt to various complex samples, such as body fluids, tissues, cells, etc., making the scope of proteomics research more extensive.