Q Exactive HF High Resolution Mass Spectrometer LC-MS

Q Exactive HF High Resolution Mass Spectrometer LC-MSThermo ScientificTM Q Exactive HFTM ultra-high electrostatic field orbital trap Fourier transform bench mass spectrometry combines high-performance quadrupole rods that provide parent ion selection with ultra-high resolution and high-quality precision ultra-high electrostatic field orbital traps. With faster detection speed and higher resolution, it has better performance in applications such as peptides, proteins, and small molecules. Thermo Fisher has added an ultra-high field Orbitrap mass analyzer to the validated Q Exactive Plus LC-MS system. This system is designed for faster identification, characterization, quantification, and confirmation in a highly reliable analysis; In order to significantly improve analysis efficiency, the maximum scanning speed has been increased by 50%. The Q Exactive HF system can complete dozens of complete MSMS scans in 1 second.

Thermo ScientificTM Q Exactive HFTM ultra-high electrostatic field orbital trap Fourier transform bench mass spectrometry combines high-performance quadrupole rods that provide parent ion selection with ultra-high resolution and high-quality precision ultra-high electrostatic field orbital traps. With faster detection speed and higher resolution, it has better performance in applications such as peptides, proteins, and small molecules.

Thermo Fisher has added an ultra-high field Orbitrap mass analyzer to the validated Q Exactive Plus LC-MS system. This system is designed for faster identification, characterization, quantification, and confirmation in a highly reliable analysis; In order to significantly improve analysis efficiency, the maximum scanning speed has been increased by 50%. The Q Exactive HF system can complete dozens of complete MSMS scans in 1 second.

Q Exactive HF High Resolution Mass Spectrometer LC-MSThe ultra-high electrostatic field orbit trap Fourier transform bench mass spectrometry combines high-performance quadrupole rods that provide parent ion selection and ultra-high electrostatic field orbit traps with ultra-high resolution and high-quality accuracy. With faster detection speed and higher resolution, it has better performance in applications such as peptides, proteins, and small molecules. The scanning speed of the new generation high-resolution accurate mass LC-MS system has been increased by 50%, and the scanning speed of the Thermo Scientific Q Exactive HF system is as high as 18Hz.

Thermo Fisher Scientific has added an ultra-high field Orbitrap mass analyzer to the validated Q Exactive Plus LC-MS system. This system is designed for faster identification, characterization, quantification, and confirmation in a highly reliable analysis; In order to significantly improve analysis efficiency, the maximum scanning speed was increased from 12Hz to 18Hz. The Q Exactive HF system can complete 18 complete MS/MS scans in 1 second, compared to the previous Q Exactive system that scanned 9 times in 1 second, and there is no loss in data quality under the same time gradient conditions. The maximum resolution of m/z 200 reaches 240000.

1、 Quality Analysis and Scope

• Quality range: 50-6000 m/z; Biopharmaceutical options can reach up to 8000 m/z.

• Quality accuracy: Internal standard<1 ppm RMS; External standard<3 ppm RMS.

• Dynamic range:>5000:1.

2、 Resolution (FWHM @ m/z 200)

• Maximum resolution: 240000 (1.5 Hz).

• Common gears:

• 120,000 @ 3 Hz

• 60,000 @ 7 Hz

• 30,000 @ 12 Hz

• 15,000 @ 18 Hz。

3、 Scanning speed and performance

• Full MS: up to 18 Hz (15000 resolution).

• MS/MS (HCD): up to 18 Hz.

• Polarity switching:<1 second (at 60000 resolution, full scan in positive/negative mode once each).

• Multi channel selection: Up to 10 precursor ions can be scanned in a single scan.

4、 Sensitivity (sample on column)

• MS/MS：100 fg ，S/N=150:1。

• SIM：50 fg 利， S/N=150:1。

• Full MS：500 fg ，S/N=100:1。

5、 Ion source and fragmentation

• Standard ion source: HESI ‑ II (heating electric spray, 0 – 600 ℃) ESI。

• Fragmentation method: HCD (high-energy collision dissociation), adjustable collision energy.

• Quadrupole pole: HyperQuad conjugate hyperbolic metal molybdenum quadrupole pole, isolation accuracy ≤ 0.4 Da.

6、 Vacuum and Environment

• Vacuum system: molecular turbo pump+front-end mechanical pump.

• Working environment: temperature of 15-26 ℃, humidity of 40% -70% (non condensing).

7、 Size and weight

• Host (length x width x height): approximately 950 x 830 x 910 mm.

• Host weight: approximately 182 kg (excluding the front stage pump).

• Front stage pump weight: approximately 100 kg.

8、 Core Technology

• Ultra high field Orbitrap: The resolution is about 1.8 times higher than standard Orbitrap.

• Advanced Quadrupole Technology (AQT): Increases ion transport efficiency by ≥ 2 times.

• AABG (Advanced Active Beam Guide): reduces noise and improves stability.

• Optional complete protein mode: enhanced molecular ion capture, suitable for antibody/complete protein analysis.