Q ExACTION plus high-resolution mass spectrometer

Thermo Scientific ™ Q Exactive ™ Combination quadrupole Orbitrap ™ The mass spectrometer introduced the revolutionary concept of Quanfirmation. Q Exactive ™ Plus combination quadrupole Orbitrap ™ Mass spectrometers have taken Quanfirmation a step further with improved performance and new options, enhancing its application capabilities in various fields from reliable qualitative/quantitative screening studies to complete characterization of monoclonal antibodies.

Advanced quadrupole technology (AQT) improves the selection and transport of parent ions, enabling more accurate quantification of low abundance analytes in complex matrices

Complex Data Independence Acquisition (DIA) and Parallel Reaction Monitoring (PRM) provide absolutely reliable and reproducible quantitative capabilities

Advanced Active Ion Beam Transport Components (AABG) reduce noise and maintenance requirements

Q ExACTION plus high-resolution mass spectrometerperformance metrics

1. Quality range: 50~6000 m/z;
2. Instrument resolution: 280000 FWHM (m/z=200);
3. Switching speed of positive and negative ions: less than 1 second
4. Scanning modes: MS and MS/MS full scan, selected ion scan, parallel reaction monitoring sub ion scan, data dependent sub ion scan, data independent scan, full sub ion collision fragmentation scan;
5. MS/MS sensitivity: 50 fg injection S/N>1000:1;
6. Linear range:>105.

Q ExACTION plus high-resolution mass spectrometerFunctions and Applications

1. Suitable for high-resolution mass spectrometry determination of moderately polar and polar compounds;
2. Accurate mass to charge ratios of compounds can be obtained, with excellent qualitative characterization and quantitative analysis capabilities;
3. Select parent ions for online secondary mass spectrometry analysis of target components to obtain more structural information;
4. Equipped with ionization sources of electric spray ionization (ESI), nanospray and atmospheric pressure chemical ionization (APCI);
5. Equipped with ultra-high performance liquid chromatography and nanoliter liquid chromatography, it can be used for the separation and analysis of complex multi-component substances;
6. Suitable for fields such as organic synthesis, natural product analysis, biopharmaceuticals, proteomics, metabolomics, etc.

Sample Requirements

1. Both liquid and solid samples are acceptable;
2. Suggest preparing a solution with a concentration level of 0.5 mg/mL;
3. It is recommended to use methanol, acetonitrile, water, or a system that is compatible with the above solvents for solvent preparation;
4. The sample does not contain strong acids, strong bases, or non-volatile salts.