Pseudomonas aeruginosa CMCC (B) 10104 (quantitative strain)

Expected useThis product is a freeze-dried product containing a certain amount of microorganisms. As a quantitative quality control sample.

Main componentsThe main components are bacterial strains and freeze-drying protectants.

Source of strain: The supplier of standard strains is international and national strain collection centers, with a passage count of ≤4.

Storage conditions and expiration date-20 ℃ and below, expiration date12month

【 Usage 】

1.Flat plate coating method:

(1) Before use, balance the unopened penicillin bottle to room temperature.

(2) Aseptic operation: Open the quantitative strain bottle stopper, use a pipette to aspirate 1.1mL of the complex solution into the penicillin bottle, cover the bottle stopper, and vortex and shake5～10sMake the ball completely dissolved.

(3) Use a pipette to aspirate 100 μ mLSpread the bacterial solution evenly onto the test medium and control medium using a coating rod.

(4) Invert the plate and cultivate it under suitable conditions.

2. Liquid inoculation or pouring dish method:

(1) Before use, balance the unopened penicillin bottle to room temperature.

(2) Aseptic operation: Open the quantitative strain bottle stopper, use a pipette to aspirate 1.1mL of the complex solution into the penicillin bottle, cover the bottle stopper, and vortex and shake5～10sMake the ball completely dissolved.

(3) Use a pipette to aspirate1mLMicrobial solution, add9mLMix well in sterile saline solution.

(4) Suck the above bacterial solution, add it to the test liquid culture medium and counting or reference culture medium, and incubate under suitable conditions. Or add it to a sterile empty petri dish, inject 15mL to 20mL of melted test medium with a temperature not exceeding 46 ℃, mix well, and cover with 5mL of medium after the medium solidifies,Invert and culture after solidification.

Pseudomonas aeruginosa CMCC (B) 10104 (quantitative strain)Appraisal result:

1. The colony characteristics on the hexadecyltrimethylammonium bromide plate are green colonies, as shown in Figure 1.

Figure 1 inCharacteristics of bacterial colonies on cetyltrimethylammonium bromide agar plates

2. Oxidase test strip: The result is purple and positive.

Figure 2 Results of oxidase test

3. Gram staining microscopy: Gram negative bacteria.

Figure 3 Gram staining microscopy

4. Acetamide broth: produces a brick red precipitate (as shown in Figure 4); The culture medium (PDP) used for the determination of pyocyanin appears pink in the hydrochloric acid layer (as shown in Figure 5); Kim's B medium: produces fluorescence (as shown in Figure 6).

Figure 4 Acetamide broth	Figure 5: Culture medium (PDP) for the determination of pyocyanin	Figure 6 Kim's B medium

5. Biochemical testing of the Bojian Gram negative bacteria identification system: Pseudomonas aeruginosa.

Figure 7 Biochemical detection results of Bojian Gram negative bacteria identification system

【Pseudomonas aeruginosa CMCC (B) 10104 (quantitative strain)Product performance indicators

This product is a freeze-dried water-soluble ball containing a certain amount of microorganisms. The ball is a white approximately spherical shape. After cultivation, there should be no growth of miscellaneous bacteria.