Pancreatic casein soy peptone agar medium (TSA) (ready to use bagged)

Pancreatic casein soy peptone agar (TSA) (ready to use bagged) product features:

This product is a bagged ready to use culture medium newly launched by Haibo. It is a sterilized finished culture medium that can be directly used for preparing plates after being heated in a water bath.

Compared to dry powder culture medium, it eliminates the tedious preparation processes such as weighing, dissolution, and sterilization, and is more convenient and efficient to use;

Compared to ready to use flat culture media, it has the advantages of simple packaging, easy transportation, low pollution, and low cost.

Purpose:A universal nutrient medium used for the cultivation of various microorganisms.

Advantages:No need for complicated preparation of culture medium, it can be directly used for preparing plates after heating in a water bath.

Ingredients (g/L)

Inspection principle:

Tryptone (casein hydrolysate) and soybean papain hydrolysate provide nitrogen sources, vitamins, and growth factors; Sodium chloride maintains a balanced osmotic pressure; Agar is a coagulant for culture media.

Storage conditions and expiration date

1.2-25℃ 保存。

2. This product is valid for six months under storage conditions.

Usage:

Step 1 [Re solution Heating]：Immerse the unopened HBBD medium (retaining the outer packaging bag) pellets completely in a 100 ℃ boiling water bath.

Heating method:

Recommended to useInduction cooker+stainless steel pot/stainless steel bucketWait for the container (add water until the pill is fully submerged in the bag).Or use a laboratory water bath (note slow heating, it is recommended to preheat in advance)

·Container during heatingWuwan fully sealedTo avoid leakage of liquid inside the bag due to increased pressure.

·OptionalPerforatedContainer lid, orLeave some gapsExhaust - see video for details.

Step 2 [Dissolve]：After heating continuously for 20-30 minutes, gently shake the bag to confirm that the culture medium is completely dissolved (uniform liquid without clots).

Step 3 [Cooling]：Take out the bag and let it cool naturally to 45 ℃~50 ℃ (slightly hot to the touch but not too hot).

Step 4 ：In a sterile environment, tear open the outer packaging, unscrew the bottle cap, slowly pour the culture medium into a sterile petri dish, let it stand and solidify before use.

Precautions

If there is pollution, damage, discoloration, leakage, etc., it cannot be used.

During the water bath heating process, there is no need to open the outer packaging. It is normal for a small amount of gas inside the product to expand when heated.

3. After use, the culture medium should be disinfected.

4. The usage method of this product is not mandatory. If the user has specific reference materials, corresponding adjustments can be made.

Staphylococcus aureus There is yellow pigment				Escherichia coli Colorless and transparent large bacterial colonies
Salmonella typhimurium Colorless large bacterial colony				Pseudomonas aeruginosa There is green pigment
Bacillus subtilis White irregular colony				Candida albicans White colony
Black mold White mycelium

Pancreatic casein soy peptone agar medium (TSA) (ready to use bagged)(200ml) (Pharmacopoeia)Microbial sensitivity test:

Prepare the culture medium according to the label instructions and inoculate the following quality control strains,Incubate aerobic at 30-35 ℃ for 18-24 hours.