Organizational fixative FM Fix

Maintaining tissue morphology and antigen stability, with excellent staining properties

Organizational fixative FM Fix

Organizational fixation is a key step in protecting biological samples, which can effectively prevent degradation caused by sample self decomposition or decay, and can stably maintain the protein and other components that make up the tissue. In tissue fixation, formalin fixation is widely used in scientific research such as microscopy specimens and pathological examinations, but it can affect the three-dimensional structure or antigen stability of some proteins, leading to problems such as decreased staining.

FUJIFILM Wako has developed FM Fix, a fixative that maintains tissue morphology and antigen stability with excellent tissue staining properties, based on the technology developed by Professor Takahashi and Professor Kou of the Department of Nephrology at Nagoya University.

◆Organizational fixativeFunction and Problems

Tissue fixation is an important operation to protect biological samples from degradation caused by self decomposition and decay, and can stably maintain the protein and other components that make up the tissue. Among the methods of tissue fixation, formalin fixation is widely used in microscopy specimens, scientific research, and pathological examinations. However, this method can affect the three-dimensional structure and antigen stability of some proteins, leading to a decrease in staining. Therefore, it is necessary to select the fixative according to the experimental purpose.

◆ Product Overview

FM Fix is a fixative that can maintain tissue shape, antigen stability, and excellent tissue staining properties. This product is suitable for various purposes of tissue fixation.

◆ FM Fix (L) for organizational fixation

This product is a fixative that exhibits excellent performance in maintaining tissue morphology, protein stereostructure, and tissue staining. After tissue fixation, the three-dimensional structure of proteins is easier to maintain and can stably detect antigens such as complement. In addition, due to its characteristics including the difficulty in weakening fluorescent protein signals such as GFP, it is suitable for tissue transparency.

characteristic

● Can maintain tissue morphology and antigen stability, with excellent staining properties

In complement fluorescence immunostaining, freezing has a staining effect that surpasses acetone fixation

After fixation, fluorescent protein signals such as GFP are not easily weakened

● Suitable for organizational transparency technologyIn SeeDB2The organization is fixed

● Suitable for fixation during RNA detection using in situ hybridization (ISH) technology

experimental data

▼Immunohistochemical staining of mouse femur

<Data provided>Professor Takahashi from the Department of Pathology and Nephrology, Graduate School of Medicine, Nagoya University

Fluorescence labeled anti-Sca1 antibody and Isolactin GS-IB4 were intravenously injected into mice, and after 1 hour, FM Fix (L) was used for perfusion fixation, followed by removal of the femur. The collected samples were fixed in FM Fix (L) fixative at 4 ° C under light shielding conditions for 2 days. Afterwards, decalcification and sucrose treatment were performed, and the mixture was embedded with O.C.T embedding agent and stored at -30 ° C. Finally, perform a thin cut and observe the entire skeletal structure.

◆ FM Fix (s) for organizational fixation

This product is an optimized fixative specifically designed for small tissue samples such as needle aspiration biopsy and tissues with good permeability.

characteristic

Suitable for small tissues such as needle aspiration biopsy and tissues with good permeability

● Can maintain tissue morphology and antigen stability, with excellent staining properties

The frozen tissue fixed with this product can be used for Periodic Acid Schiff (PAS) staining, Periodic Acid Methenamine Silver (PAM) staining, and Masson Trichrome (MT) staining after thawing.

Application Data

▼Tissue staining of human kidney biopsy (HE, MT, PAM, PAS staining)

<Data provided>Professor Takahashi from the Department of Pathology and Nephrology, Graduate School of Medicine, Nagoya University

Collect kidney tissue using a 16G biopsy and fix it with FM Fix (s) at 4 ° C for 1 day. Subsequently, paraffin embedding and sectioning were performed, followed by hematoxylin eosin (HE) staining, periodic acid Schiff (PAS) staining, hexamine silver periodate (PAM) staining, and Masson Trichrome (MT) staining in sequence.

【result】

Compared with 10% neutral formalin, FM Fix (s) better maintains the structure of tissues

▼CX3CR1-GFPFluorescence immunohistochemical staining of mouse kidneys

<Data provided>Professor Takahashi from the Department of Pathology and Nephrology, Graduate School of Medicine, Nagoya University

Intravenous injection of 30 µ g Isolectin GS-IB4 Alexa Fluor into CX3CR1-GFP mice^®647, remove the kidney after 30 minutes. Subsequently, cut the kidney into small pieces of about 2 mm using a razor blade and fix it with this product (24 hours, 4 ° C, away from light). The fixed tissue was replaced with a 30% sucrose solution, embedded with O.C.T. embedding agent, and stored at -30 ° C. Then, thin slice with a frozen slicer, air dry for 1 hour, and clean with 0.1% Triton. Finally, seal the film with DAPI sealing agent and observe it under a microscope.

*Alexa Fluor^®It is a registered trademark of Invitrogen

【result】

In FM Fix (s) fixative, the fluorescence signal of GFP protein remains undiminished and can be directly detected without the need for anti GFP antibodies

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