NatureThink Aortic Blood Flow Stimulating Cell Culture Instrument

Product Introduction

The aortic valve is a half moon valve located between the left ventricle and the aorta, mainly used to regulate the unidirectional flow of blood between the left ventricle and the aorta. Aortic calcification is a common aortic valve disease, and the formation of aortic valve calcification is influenced by mechanical factors and mechanisms in the blood flow. The mechanical factors involved in aortic stenosis are quite complex, mainly including blood flow shear force, pressure, and tensile force. Shear force is one of the important factors leading to aortic calcification.

NatureThink Aortic Blood Flow Stimulating Cell Culture InstrumentUsed for in vitro simulation of physiological and non physiological shear stress stimulation environments, it helps to understand the role of shear force in the aortic valve and its potential role in the pathogenesis of aortic stenosis. The system has three fluid modes: constant current shear stress, pulse shear stress, and oscillation shear stress. It is possible to compare the effects of steady-state flow and oscillatory flow on endothelial cell function, or the effects of steady-state flow and pulsatile flow on endothelial cell function. Experimental studies on the efficacy of endothelial cell drugs can be conducted. Corresponding experimental research can be conducted according to different needs, mainly applied to in vitro culture of cells, tissues, organoids, and organs. More experiments can be adjusted according to users' own needs to simulate real in vivo environments.

NatureThink Aortic Blood Flow Stimulating Cell Culture InstrumentBy controlling the pump system through a computer system, important parameters such as flow rate, shear force, and time can be set independently; The fluid device can be placed in the incubator for use or used separately; Widely applicable and suitable for interdisciplinary fusion experimental research.

Parameter Description

• Cultivation area: 64 square centimeters;

• Reusable: The part of the instrument in contact with the culture medium can be sterilized under high temperature and pressure;

• Fluid shear force modes: steady flow, pulsating flow, oscillatory flow;

• Four channel culture: Each channel can be used for different cell cultures;

• User defined time, fluid shear force, and direction, etc.

  
  

Product Advantages

• Used for adherent cells;

• Long term cell culture;

• Multiple shear force stimulation modes;

• The ratio of cultivation area to culture medium is small;

• Widely applicable and suitable for long-term cultivation of adherent cells;

• The cell culture area is large, meeting the demand for protein extraction;

• The contact area of the culture medium can be sterilized and disinfected under high temperature and pressure.

  
  

Possible application research：

1. Experimental simulation of the effect of fluid shear force on cell stimulation in mechanically sensitive ion channels.

2. Simulate experiments on inducing differentiation of bone marrow mesenchymal stem cells into different cells under different shear stress stimuli.

3. Experimental simulation of the effect of shear stress stimulation in interstitial flow on osteoclast migration.

4. Experimental simulation of drug cell tissue interactions under blood flow shear stress.

5. The relationship between circulating tumor cells stimulated by simulated blood flow shear force and the occurrence and development of breast cancer.

6. Simulate the effects of blood flow shear force on the proliferation, invasion, migration, drug sensitivity, and survival of circulating tumor cells in the blood.

7. Experimental simulation of the effect of blood flow shear force stimulation on the morphology of cancer cells.

8. Experimental simulation of the effect of blood flow shear stress stimulation on the migration of liver cancer cells.

9. Experimental simulation of the effect of blood flow shear stress stimulation on tumor cell metastasis.

10. Experimental study on epithelial mesenchymal transition and ovarian cancer cell metastasis under simulated blood flow shear stress in ovarian cancer.

11. Simulate the phagocytic response of astrocytes under blood flow shear stress conditions.

13. Simulate the effect of blood flow shear stress stimulation on endothelial function.

14. Simulate the effect of blood flow shear stress stimulation on inflammatory response.

15. Simulate the effect of blood flow shear stress stimulation on the function of the glycocalyx barrier.

16. Simulate the morphological changes of cells under the stimulation of blood flow shear force.

17. Simulate the effect of fluid shear force on bone remodeling during orthodontic procedures.

18. Study on the mechanism of simulated blood flow shear stress stimulation and venous thrombosis formation.

19. Simulate the effect of blood flow shear stress stimulation on the proliferation and migration of corneal cells.

20. Simulate the effect of blood flow shear force on the cytoskeleton of cochlear vascular endothelial cells.

21. Study on Fluid Dynamics Factors Related to Skeletal Diseases

• Joint problems: osteoarthritis, ankylosing spondylitis, etc; The effect of shear force stimulation on cartilage matrix metabolism and chondrocyte proliferation in joint chondrocytes is crucial for the repair of joint cartilage injuries.

• Skeletal problems: osteoporosis, osteoporosis, fragility fractures, traumatic fractures, etc; Shear force is the main stress that skeletal cells can perceive, and its effect on skeletal cells and the basic structure of bones is of great significance.

• Orthodontic issues: jaw deformities; Orthodontics, etc; Mechanical force stimulates the release of extracellular matrix related factors to participate in periodontal physiological functions, ensuring sustained bone resorption activity.

• Muscle problems: connective tissue diseases, muscle atrophy, etc; ……

NaturethinkWe are one of the earliest domestic enterprises engaged in the research and sales of biomimetic cell culture instruments. Years of technological accumulation have enabled us to have independent research and development capabilities and core technologies in the field of human biomimetic environment cultivation; At the same time, we provide users with instrument and equipment improvement, design, and research and development services.

We promise that all products sold are independently developed and do not infringe on intellectual property rights. Users can use them with confidence

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