Microplate respiration measurement system series

Research on Small Organisms

If you are studying the oxygen consumption rate of small organisms such as eggs/embryos/larvae of fish or invertebrates, copepods, or water fleas, you may consider a closed 24 well microplate respiration measurement system.

Microplate respiration measurement system

MICROPLATE SYSTEM

（Core model24通道/ 80microliter, Scalable）

Overview

The microplate small animal respiration measurement system is a high-throughput, automated respiratory metabolism detection device designed specifically for microorganisms and small organisms. It uses non-invasive fluorescent oxygen detection technology to monitor the oxygen consumption/production rate of samples in sealed micropores in real time and accurately capture weak respiratory signals.

This microplate respiration measurement system is easy to operate and has high flux. It can measure the individual respiration rate of micro aquatic, terrestrial, and microbial organisms over a wide temperature range (5-40 ℃). After the entire system is loaded into the standard hard transport box, it is easy to transport and can be sent to remote field workstations and other places. The system supports multi environment detection of water, air/gas, and can expand from 24 channels to 240 channels. It is widely used in research fields such as aquatic biology, microbiology, environmental toxicology, and plant physiology.

research subject

Objects suitable for microscale metabolic research:

• Aquatic microorganisms: zebrafish/medaka embryos/juveniles, Xenopus eggs and tadpoles, water fleas, copepods, etc

• Terrestrial microorganisms: mosquitoes/ticks, spiders, arthropod pupae, fruit flies such as Drosophila melanogaster, terrestrial chironomid larvae, etc

• Microorganisms/Single Cells: Bacteria, Algae, Yeast, Protozoa, Cell Suspension

• Plant samples: various plant seeds (Arabidopsis/rice/wheat/corn), ex vivo plant tissues

• Other: Ex vivo animal tissues/organs, egg sacs

As long as it can be inserted into the pores of a glass microplate, the oxygen consumption (or oxygen generation) rate of almost all organisms can be measured by this system.

Product Features

u Non invasive non-destructive testing:Optical fluorescent oxygen sensor, does not come into contact with the sample, can monitor continuously for a long time without damaging biological activity.

u High throughput flexible expansion:The basic model is equipped with a 24 hole glass microplate, and a single computer can be extended to connect up to 10 24 hole plate readers for high-throughput detection; Multiple pore volumes available: 80, 200, 500, 940, and 1700 μ L

u High precision and stable measurement:Real time detection of dissolved oxygen content in each airtight hole (closed breath measurement method), which can measure the oxygen consumption rate or generation rate in water or gas phase. The sensor response time is less than 30 seconds, the drift is less than 1% of air saturation per week, and it accurately captures weak metabolic signals.

u Automated full process operation:MicroResp adapted for Windows 11 system ™ Specialized software, easy to operate, supports data recording, statistics, and analysis

u Multi scenario environment adaptation:Compact design, suitable for use in incubators/water baths or on shaker tables, supports temperature control of 5-45 ℃, and the entire set can be easily transported to field stations.

u Low consumable cost:The optical dissolved oxygen sensor can be reused and can be calibrated multiple times before conducting experiments; Borosilicate glass microplates can be reused.

System Composition

n core hardware

· 24 Channel microplate reader (including splitter and power adapter)

· MicroResp™ V1Software (including authorized encryption)

· 24 hole borosilicate glass microporous plate (multiple specifications to choose from)

The core microplate system comes standard with a borosilicate glass microplate, providing the following five specifications of microplates that are compatible with the same reading instrument that comes standard with the system. With just one set of microplate system, microplates with different pore volumes can be replaced, making it easy to study organisms of different body types and species.

The inner diameter and depth parameters of each specification are shown in the table below：

n Sealing and auxiliary accessories

· Micro porous plate guide device (precise alignment)

· Silicone pad, compression block (achieving airtight sealing)

· Airtight self-adhesive sealing film, sealing roller (auxiliary sealing)

n Portable protection

· Hard transport box (for storage and portable transportation of complete equipment)

Real time acquisition of oxygen data

· Through MicroResp ™ Microplate respiration measurement system, real-time calculation and analysis of metabolic rates of water fleas, fruit flies, mosquitoes, zebrafish, and other models or microorganisms. MicroResp ™ Responsible for collecting oxygen data from microplate readers and automatically calculating MO2/VO2 data for each hole on the glass plate.

The actual oxygen content of each hole is displayed in the numerical view or presented in an independent form in a customizable data graph

· To obtain a more comprehensive overview, all data trajectories can be monitored in real-time composite graphs and labeled with applied processing operations. The data graphs from each experimental process can be quickly exported as images or Excel files for easy presentation of results before data analysis

· MicroResp ™ Not only can it monitor the oxygen consumption in each well position, but it can also monitor the generation of oxygen. Switching measurement modes between water bodies (including freshwater and saltwater) and air is easy to operate, just like flipping a switch.

technical parameters

application field

1. Plant Physiology: Detection of Seed Germination, Respiratory Metabolism, and Study on Plant Stress Response

2. Aquatic Biology: Detection of Oxygen Consumption Rate of Aquatic Organisms and Study on Metabolic Patterns of Embryonic Development

3. Microbiology: Microbial respiratory activity detection, metabolic inhibitor efficacy evaluation

4. Environmental Toxicology: Effects of Pollutants on Biological Metabolism, Biological Testing of Water Quality

5. Drug screening: screening of the metabolic effects of high-throughput drugs on microorganisms

6. Ecological research: Field biological metabolism detection, polar biological adaptation mechanism research

Simple experimental operation

n Place single or multiple experimental organisms into airtight glass wells of 80-1700 microliters each, typically using pipettes, scrapers, or pipettes (when dealing with escaping arthropods);

n Using an airtight, transparent, and self-adhesive polyester sealing film attached to the surface of the glass microplate to seal each hole.Glass material has impermeabilityOrdinary plastic microplates, on the other hand, become a "storage" or "release source" of oxygen based on the oxygen partial pressure of the sample, which affects the detection results;

n Place the sealed glass microplate on the reading instrument through the positioning guide, aligning the 24 dissolved oxygen sensing points with the 24 blue LED light sources and receivers of the reading instrument accurately. The reading instrument, positioning guide, and microplate can be easily placed in the incubator or directly placed in the constant temperature chamber to achieve temperature control of the glass microplate.

optionalTemperature control scheme

n Optional acrylic flow-through water bath for temperature control. Place the water bath on the reading instrument and place the sealed glass microplate into the water bath. A single glass microplate is fixed in the appropriate position, and 24 oxygen sensor points are directly concentrated on 24 LED light sources to obtain the best signal intensity. The water bath port is used to measure aquatic respiration rate at any ambient temperature or during calibration by freezing/heating water flow on an immersion glass microplate.

n Replace the temperature probes of Witrox 1 and Witrox 4 instruments with 5-meter-long shielded cables. The tolerance of PT 1000 sensor is 1/3 of that of Class B sensor, with high accuracy of+/0.15 ° C.

Anti dissolved oxygen layering treatment

For non motile organisms in the aqueous phase, such as embryos, hydroids, sea anemones, or microorganisms, an orbital shaker can be used to reduce the risk of dissolved oxygen stratification in the pores.

Cleaning of sensors and microplates

Each detection well is equipped with a non-invasive and reusable dissolved oxygen sensing point, which uses dynamic fluorescence quenching method to detect dissolved oxygen. After the experiment, the microplate can be quickly cleaned with mild soapy water and deionized water, or sterilized with ethanol or mild bleach for easy reuse in subsequent experiments.

Compact design, convenient transportation

When using this system, it occupies very little laboratory space and only requires one wall power outlet for power supplyUSBConnect the interface to a Windows system computer. After being loaded into the standard hard transport box, the entire system can be safely transported to various places, including remote areas.

Application Case Reference

The research team carried this microplate system to Antarctica to study the metabolic characteristics of terrestrial mosquito larvae

To better understand how insects adaptRegarding the environment, a group of researchers has been studying Antarctic midges. The team is led by Nicholas Teets (Associate Professor at the University of Kentucky) and includes Jack Devlin (PhD student at the University of Kentucky), Cleverson de Sousa Lima (PhD student at the University of Kentucky), Yuta Kawarasaki (Associate Professor at Gustavus Adolophus College), JD Gantz (Assistant Professor at Hendrix College), and Vitror Pavinato (postdoctoral fellow at the University of Kentucky). Researchers at Ohio State University are studying how this insect affectsThe molecular and physiological mechanisms behind stress tolerance, and how it survives in harsh Antarctic conditions. As part of their research, the team recently completed a field survey season on the Antarctic Peninsula, where they collected terrestrial insects from various islands and conducted physiological experiments on research ships and stations. One of the main interests of the research team is how these insects cope with the energy challenges brought about by stress.

Cleverson Lima uses MicroResp ™ A microplate respiration measurement system for software. The microporous glass plate is placed in an acrylic water bath (near Cleverson's left hand), and a recirculation bath provides water flow of 10 ° C through the bath to maintain temperature stability.

Using Loligo ® The microplate respiration measurement system from Systems is capable of measuring real-time oxygen consumption of individual terrestrial midge larvae under various environmental pressures, data that the team was unable to obtain in previous projects. Their new tool, the microplate respiration measurement system, enables researchers to measure metabolic rate in real-time and provide key data on how insects are affected by different pressure sources.

MicroResp ™ v1.1， The built-in software of the microplate system supports real-time and timestamp MO2 calculation for measuring air/gas and water.

Team member Jack Devlin is interested in the potential impact of microplastic pollution on Antarctica. He also used a microplate respiration measurement system to investigate whether exposure to microplastics would cause changes in metabolic rate. These research findings are crucial for understanding the potential impact of microplastic pollution on fragile ecosystems on the African continent.

MicroResp ™ The MO2 and oxygen time data plots created by Jack Devlin were used for each individual glass plate well containing Antarctic land midge larvae. Black is blank, gray is the control group, and blue is insects exposed to high concentrations of microplastics. Maintain humidity levels by adding a damp filter paper to each well.

The research team also compared the tolerance and genome of Belgian worms in Antarctica with several close relatives to determine the key mechanisms by which this insect thrives in Antarctica. This study can better understand how insects adapt to their environment and provide insights into species' ability to adapt to constantly changing environments.

Peer reviewed papers published using this system

The following are some published papers citing this microplate respiration measurement system:

1. Effects of temperature on metabolic rate during metamorphosis in the alfalfa leafcutting beeThe effect of temperature on the metabolic rate of alfalfa leaf cutting bees during their developmental stages

Kayla N. Earls, Jacob B. Campbell, Joseph P. Rinehart, Kendra J. Greenlee (2023)Biology OpenLink to article

2. Effects of elevated CO2 on metabolic rate and nitrogenous waste handling in the early life stages of yellowfin tuna (Thunnus albacares) The effect of high concentration of carbon dioxide on the metabolic rate and nitrogen-containing waste metabolism of yellowfin tuna in the early life history stages

Rachael M. Heuer, Yadong Wang, Christina Pasparakis, Wenlong Zhang, Vernon Scholey, Daniel Margulies, Martin Grosell (2023),Comparative Biochemistry and Physiology Part A: Molecular & Integrative PhysiologyLink to article

3. Chemical manipulation of mitochondrial function affects metabolism of red carotenoids in a marine copepod (Tigriopus californicus) The effect of chemical regulation of mitochondrial function on the metabolism of red carotenoids in marine copepods (California tiger spotted water fleas)

Matthew J. Powers, James A. Baty, Alexis M. Dinga, James H. Mao, Geoffrey E. Hill (2022),Journal of Experimental Biology.Link to article

4. Metabolic responses to crude oil during early life stages reveal critical developmental windows in the zebrafish (Danio rerio) Metabolic response to crude oil exposure reveals key window period for early development of zebrafish

Karem N. Vazquez Roman, Warren W. Burggren (2022), Comparative Biochemistry and Physiology Part C: Toxicology & Pharmacology.Link to article

5. Effects of marine mine tailing exposure on the development, growth, and lipid accumulation in Calanus finmarchicusEffects of exposure to marine slag on the development, growth, and lipid accumulation of Arctic water fleas

Linn H. Svendheim, Tjalling Jager, Pål A. Olsvik, Ida Beathe Øverjordet, Tomasz M. Ciesielski, Trond Nordtug, Torstein Kristensen, Bjørn Henrik Hansen, Bjarne Kvæstad, Dag Altin, Julia Farkas (2021) ,Chemosphere.Link to article

6. Water-soluble fraction of crude oil affects variability and has transgenerational effects in Daphnia magnaThe impact of water-soluble components in crude oil on the variability and transgenerational effects of Daphnia magna

Mikko Nikinmaa, Emilie Suominen, Katja Anttila (2019), Aquatic Toxicology.

Link to article

7. Genetic Variation in Metabolic Rate and Correlations with Other Energy Budget Components and Life History in Daphnia magnaGenetic variation of metabolic rate in Daphnia magna and its correlation with other energy budget components and life history characteristics

Sigurd Einum, Erlend I. F. Fossen, Victor Parry & Christophe Pélabon (2019), Evolutionary Biology.Link to article

8. Ecotoxicological assessment of wastewater treated by the novel solar chlor-photo-Fenton process for sustainable crop irrigationNew type of solar chlorine-Ecotoxicological evaluation of wastewater treatment using the Fenton process for sustainable agricultural irrigation

Belachqer-El Attar, S; Taborelli, P; Soriano-Molina, P; Roslev, P; Pérez, JA Sánchez (2026),Journal of Environmental Management.

Microplate respiration measurement system series

Origin: Loligo, Denmark