Core Resting Breathing Measurement System (Fish& Invertebrate movement, etc

Core resting breathing measurement system (fish&invertebrates, etc.)Introduction

The Core Resting Respirometry System is from Loligo, Denmark ® A research grade respiratory measurement system developed by Systems for fully automated measurement of resting oxygen consumption rate (O ₂ consumption rate) in aquatic organisms (fish, invertebrates, etc.). The system was jointly developed by the University of Copenhagen and Aalborg University in Denmark and can be used to measure the oxygen consumption of fish, aquatic invertebrates, fish eggs and their embryos, and even plankton.

The system adopts the "intermittent" breathing measurement method, combining the advantages of "open" and "closed" measurement methods, and can achieve long-term continuous measurement with high time resolution. The system configuration includes a multi-channel fluorescent fiber optic oxygen measurement host, static or dynamic breathing chambers, submersible pumps AutoResp ™ Components such as automatic control and analysis software are used for studying the standard/routine metabolic rates of fish and invertebrates of various sizes. Widely used in the ecology of aquatic organisms such as freshwater fish, toxicology of aquatic environments, aquaculture, behavioral and physiological ecology of fish, developmental ecology of aquatic animals, and research on aquariums.

Core positioning and purpose

² Core functions:Fully automatic measurement of oxygen consumption rate (MO ₂) of aquatic organisms in a resting state, used to evaluate metabolic rate, energy consumption, physiological status, environmental stress response, etc.

² Applicable to:Various aquatic organisms such as fish, crustaceans, mollusks, aquatic insects, plankton, etc.

² Application Scenario:Aquaculture, fish physiology, ecotoxicology, environmental science, climate change impact research, etc.

characteristic

l Aquatic organisms using a 4-channel/4-chamber resting respiration measurement system

l Fully automatic computerized intermittent flow breathing measurement

l frontAutoResp ™ 3 software for Windows 11 including

l In depth analysis, statistics, and data export

l Build high-throughput experiments with multi-channel settings (up to 20 rooms!)

l Can be used in freshwater and full strength seawater

l Real time oxygen consumption measurement and analysis

multi-channel=High throughput

choice4roomCORESystem, additional system expansion channels can also be added/The number of rooms can be configured at once8,12,16or20A room, and all of these can be from a singlePCUp operation.

The basis of a resting breathing measurement system isfrontThe software solutionAutoResp™3

AutoResp™3It allows you to quickly initiate aquatic intermittent respiration measurements and automatically perform resting room experiments, including real-time data acquisition, sensor calibration, hardware control, water quality regulation, and data post analysis, statistics, and export.

Oxygen consumption of small organisms

Minimum chamber size range: inner diameter（ID）9-28mmThese chambers are made of borosilicate glass and come in various standard lengths, with a maximum volume of199mLThe glass chamber is composed of a cavity tube and a cavity plug, making it easy to contact/Remove living organisms.

n Each glass chamber is installed on an acrylic bracket with four slots. Fix the chamber in the appropriate position and have fixtures for external fiber optic cables, even if it is kept above the internal oxygen sensor point for non-invasive optical oxygen measurement through glass walls.

n The bracket is installed at the bottom of the acrylic water bath to maintain temperature stability in the chamber throughout the entire experimental process. A miniature impeller pump is fixed on the outside of the water bath on both sides of each chamber. One pump is used to flush the chamber (during the open phase), and the other pump is used to recirculate the water in each chamber during the closed measurement phase. Including all necessary pipes and fittings.

n Each chamber has a thin planar oxygen sensor point for non-invasive oxygen measurement.WitroxThe high-precision oxygen instrument uses wireless Bluetooth communication and then collects oxygen data from each chamber using fiber optic cables. high precisionPT1000The temperature sensor is placed in a water bath for real-time temperature compensation of oxygen data.

n The flushing pump and recirculation pump are connected through four relays/Connect the socket to ourPowerX4Power strip, through wired connectionLAN/Open software drivers via Ethernet or wireless Bluetooth/Close control and device monitoring. This allows forAutoResp™3Independently control each pump.

n WitroxandPowerX4Connect to via Bluetooth (or LAN)PConAutoResp™3Therefore, you canPCPlaced in a separate room, away from all water hazards, and directly inAutoResp™3Collect data for in-depth analysis.

Oxygen consumption of large organisms

Large chamber made of transparent acrylic, size rangeID42 -240mmThese chambers also come in various standard lengths, providing0.1 -45.2 LThe volume range. The acrylic chamber consists of one chamber tube and two chamber covers, which can be easily accessed/Remove the organisms on both sides.

n Acrylic breathing chambers are typically placed in water tanks (not included) for temperature control. The square lid means that each cavity is firmly fixed at the bottom of the can.

n Submersible pumps are placed on both sides of the chamber, providing flushing and recirculation options. Including all necessary pipes and fittings. Outside each chamber is a flowing oxygen microsensor unit that uses the same thin planar oxygen sensor point for non-invasive oxygen measurement. The flow-through oxygen cell is integrated into the recirculation pipe, and oxygen data is transmitted through fiber optic cablesWitroxThe temperature compensation of oxygen data can be achieved byWitroxofPT1000The temperature probe is kept inside the water tank to achieve this.

n The flushing and recirculation submersible pump is connected to ourPowerX4, allowed to pass throughAutoResp™3Automatic software driver controls each pump.

Origin: Lolig, Denmark