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KTA4001 Mitochondrial Membrane Potential Analysis Kit (JC-1)
NegotiableUpdate on 05/06
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Overview
The KTA4001 Mitochondrial Membrane Potential Analysis Kit (JC-1) subfamily utilizes JC-1, a fluorescent, lipophilic, cationic dye optimized for flow cytometry or fluorescence microscopy, and contains CCCP (a potent mitochondrial oxidative phosphorylation uncoupling agent)
Product Details
KTA4001 Mitochondrial Membrane Potential Analysis Kit (JC-1)
Product Information
| Product English Name | Mitochondrial Membrane Potential Assay Kit (JC-1) |
| Experimental suggestions | Abbkine's mitochondrial membrane potential detection kit (JC-1) provides a simple method for detecting changes in mitochondrial transmembrane potential based on JC-1, distinguishing between healthy and apoptotic cells |
Product attributes
| Kit components | JC-1 dye • CCCP (10 mM) Experimental buffer (5 x) |
| Features&Advantages | As an indicator of cellular health, measure mitochondrial membrane potential. • Use JC-1, a fluorescent, lipophilic, cationic dye. Red fluorescence indicates healthy mitochondria, while green fluorescence indicates mitochondria with poor health. Optimized for flow cytometry or fluorescence microscopy. • Contains CCCP (a potent mitochondrial oxidative phosphorylation uncoupling agent). |
| Precautions during use | JC-1 has low solubility and is difficult to prepare. Before use, ensure that JC-1 * is thawed and warmed to room temperature. JC-1 has photosensitivity. Incubation needs to be carried out in the dark. |
| Save Suggestions | Please refer to the storage conditions for each component in the instruction manual. From the date of shipment, it should be stable at the recommended temperature for at least 12 months. |
| transport conditions | Blue Ice Transport |
| Warning | The products listed in this article are for research purposes only and are not suitable for human or clinical diagnosis. The applications recommended by our product are not intended to violate any license by using our product. We do not assume any responsibility for any infringement or other violations that may occur when using this product. |
Additional information
| background | The decrease in mitochondrial membrane potential is a hallmark event in the early stage of cell apoptosis. The transition of JC-1 from red fluorescence to green fluorescence can easily detect the decrease in cell membrane potential, and the transition of JC-1 from red fluorescence to green fluorescence can also be used as an early detection indicator of cell apoptosis. |
Images and explanations
Fig. Hela cells stained with Abbkine Mitochondrial Membrane Potential Assay Kit (JC-1). A: Red fluorescence indicates healthy mitochondria, B: Green fluorescence indicates mitochondria in poor health (30min incubation in 20uM CCCP).
KTA4001 Mitochondrial Membrane Potential Analysis Kit (JC-1)
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