Japan ATTO electrophoresis, gel imaging and other biochemical instruments

NegotiableUpdate on 02/04

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Nature of the Manufacturer: Producers

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Place of Origin

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Overview

ATTO product line in Japan

Product Details

1. Protein and DNA electrophoresis
1.1 Micro gel protein electrophoresis

1.2 Wide gel size protein electrophoresis
Protein electrophoresis/wide gel 8x14cm

1.2.1 Purpose and Application
1.2.1.1 30 samples can be analyzed simultaneously in one electrophoresis
1.2.1.2 Use SDS-PAGE (SDS-PAGE gel electrophoresis) gel electrophoresis technology
1.2.1.3 Use non denaturing polyacrylamide gel electrophoresis technology
1.2.1.4 The main applications include protein separation, identification, purity confirmation, molecular weight estimation, etc.
1.2.1.5 Used to confirm the purity and expression level of proteins.
1.2.1.6 can also be used for DNA electrophoresis analysis and confirmation of PCR products.

1.2.2 Characteristics
1.2.2.1 Multiple samples (60 samples/two gel) can be provided for electrophoresis for large-scale screening, and multiple samples can be compared in one gel at the same time (the same experimental conditions)
1.2.2.2 High electrophoresis speed and resolution → "High speed electrophoresis mode", which can be completed within 30 minutes (300V voltage, AC) (※ It is recommended to use e-PAGEL HR or ATTO EzGel Ace buffer solution to make gel, and ATTO EzRunMOPS buffer solution is recommended)
1.2.2.3 Can use multi-channel pipettes
The sample hole spacing is 4.5 millimeters, suitable for use with multi-channel pipettes
The length of gel is the same as that of mini gel (about 8 cm)
Electrophoresis pattern comparable to mini gel, and accurate protein stripe pattern can be obtained with enough gel length
1.2.2.5 Maintain a constant temperature with a large amount of upper and lower buffer solution to reduce the "smiling face effect" (the effect formed by the rising strips at both ends of the film)
1.2.2.6 Automatic polarity switching
(The bottom electrode automatically becomes the anode (+) to avoid failure due to incorrect electrode connection)
1.2.2.7 Convenient sample application
Visible electrophoresis tank (front can be opened)
1.2.2.8 Use special film holders for easy installation of adhesive plates

H-shaped bracket → easy installation of glass panel into the instrument
1.2.2.9 Good sealing performance to prevent buffer leakage
Softness and compactness
1.2.2.10 The film uses high-quality and durable acrylic material with refractive index shielding effect
1.2.2.11 Compatible mold: WSE-1195 multi-purpose micro flat gel mold
1.2.2.12 Compatible with pre made film: m-PAGEL

1.3 gel size protein electrophoresis

1.4 Two dimensional protein electrophoresis

1.5 Slab gel pouring device (glue injection)

1.6 Nucleic acid electrophoresis

2. Pre injected gel
2.1 Pre gel mini gel electrophoresis (polyacrylamide gel electrophoresis of protein or nucleic acid)

Polyacrylamide gel is a polymer formed by free radical polymerization of acrylamide and N, N '- methylenebisacrylamide (Bis), a crosslinking agent. Acrylamide forms a linear structure, while the crosslinking agent acts as a bridge to form a polymer (gel) with network structure. The lower the gel concentration is, the larger the pore size is, so the separation of high molecular protein can be achieved. However, Bis has low solubility, so it will form uneven gel due to the existence of agglomerates. The uneven gel has low strength and is easy to tear. Therefore, the difficult problem to deal with lies in this. ATTO has successfully developed a uniform gel prepared with a new crosslinker at low concentration. The crosslinker has higher solubility and longer binding arm than Bis. It has excellent strength, with a tensile fracture stress of about 1.7 times that of Bis. In addition, due to the large pore size, the sample can smoothly enter the network structure of gel without blocking, showing higher separation.

2.2 Precast Wide Gel wide size gel electrophoresis (polyacrylamide gel electrophoresis of protein or nucleic acid)

3. Imprinting system

4. Power supply

5. Imaging system
5.1 gel imaging system

5.2 Chemiluminescence gel imaging system

5.3 Light Source

5.4 gel imaging analysis software

5.5 Calibration reference LED light source
5.5.1 Purpose and Application
Operational qualification (OQ) verification of gel imaging system
Performance qualification (PQ) verification of gel imaging system
Regular inspection of isooptical measuring equipment of gel imaging system

5.5.2 Characteristics:
5.5.2.1 Use a reference light source for calibration, and determine the light intensity value by comparing it with the reference light source used for actual luminous flux measurement.
5.5.2.2 Stable light source, using RGB LED to adjust the total light output to the level of 10 nanowatts to 10 flying watts.
5.5.2.3 is applicable for routine inspections of imaging systems and sensitive optical measurement equipment.
5.5.2.4 Use 11 levels of pulse width modulation (PWM) control.
5.5.2.5 Supports linear testing of optical measuring equipment.
5.5.2.6 RGB color switches and light levels can be freely set.
5.5.2.7 The temperature compensation function ensures that the light intensity remains stable under changes in heat.
5.5.2.8 Due to the constant light intensity during changes in ambient temperature, it is highly suitable for repeatability testing.

6. Biochemical Luminescence Spectrometer

7. Liquid pump

8. Centrifuge/Dry Incubator (Incubator)

9. Physical monitoring instruments
9.1 Mixed monitoring system
By detecting changes in the power consumption of the mixer during the mixing/stirring process, it is possible to visualize the rheological changes of various foods, such as bread dough, whipped cream, cake batter, or chemical products.