Globe Plus TEM grid based glow discharge treatment instrument

GloQube Plus TEMCarrier network glow discharge treatment instrument

GloQube ® PlusIt is a cost-effective, compact, and easy-to-use glow discharge system designed to meet the requirements of existing configurationsTEMThe requirements of the laboratory are commonly used forTEMCleaning and surface charge treatment of grid and supporting film. Like hydrophobic (oleophilic) propertiesTEMAfter gas glow discharge treatment in an air atmosphere, the surface of the carbon supported membrane is negatively charged and hydrophilic, making it easy to disperse in aqueous solution. withTEMThe popularization of applications has increased the workload, and the use of clean and reproducible resultsTEMThe grid and support film have become more important than ever before, except for ordinary ones with carbon support filmTEMIn addition to copper mesh applications, it is also widely used for adhering carbon films, proteins, antibodies, nucleic acids, and positively charged protein molecules to nucleic acids(in compliance with:Ferritin, cytochromec)Waiting for various different requirementsTEMSample pretreatment. It can achieve four different processing results:

1）Hydrophilic and negatively charged treatment

Glow discharge is used to change the carbon film, Fanghua film, andTEMThe recognized method for surface characteristics of carbon supported membranes. Carbon is naturally hydrophobic (hydrophobic), and short glow discharge treatment can change its surface properties to hydrophilic (hydrophilic). An aqueous solution must be forced into contact with a hydrophobic (non-polar) surface, but it easily spreads on hydrophilic surfaces.

2）Hydrophilic and positively charged treatment

It is not possible to obtain "hydrophilicity and positive charge" solely through glow discharge; Usually, air atmosphere glow discharge is performed first, followed by magnesium acetate(magnesium acetate)perhaps0.1%（W/V）Post treatment of polylysine solution. Commonly used to enhance the adhesion of nucleic acids to carbon films.

3）Hydrophobic and positively charged treatment

The carbon film treated in alkylamine vapor carries a positive charge. Deposition of polymers leads to changes in membrane size. Suitable for negatively charged macromolecules, it can be used to chemically adsorb various biomolecules including proteins, antibodies, and nucleic acids.

4）Hydrophobic and negatively charged treatment

Carbon films treated in a hydrocarbon reducing atmosphere (usually with methanol) can produce hydrophobicity -veOn the surface, it can strongly adsorb positively charged macromolecules such as ferritin and cytochromeCIsoprotein family (related to mitochondrial inner membrane).

Instrument features:

•The complete system meets all your needs for glow discharge

• Short air circulation time

Dual chambers for separating air and steam treatment, with automatic switching valves between chambers to prevent cross contamination

Automatic steam delivery ensures reliable and reproducible results

The purification cycle reduces the concentration of water vapor and oxygen, ensuring excellent yield of oriented macromolecules

Adjustable slow exhaust time to minimize sample disturbance; Optional quick release for quick processing

• Safe handling of reagents: safe vapor injection method using membrane sealed bottles

Three height adjustable sample stage to ensure reproducibility of results

• Has negative and positive charge discharge modes

A program recipe that can call typical standard parameters and store them1000More than one user-defined program recipe

• Intuitive capacitive touch screen control interface, dual coreARMThe processor,16GBflash memory

Recommended application:

•TEMClean and hydrophilic carbon support film for better dispersion of samples

•Improve the adhesion and orientation of proteins, nucleic acids, and antibodies

•Preparation for research on nanoparticlesTEMCopper Mesh

•Typical samples: Fanghua film, lace carbon film, porous carbon film, continuous carbon film,QuantifoilPorous support membrane

For those who frequently undergo surface modification treatments such as hydrophilicity, hydrophobicity, positive and negative charges, etcTEMLaboratory, do not hesitate, globally renowned manufacturer of electron microscopy sample preparation equipment in the UKQURUMProduced byGloQube PlusThe dual chamber glow discharge treatment machine is worth having! Note: This product is exclusively for scientific research purposes.