Intracellular mycobacteria

Strain name: Intracellular Mycobacterium

English name:Mycobacterium intracellulare‍

Ammonium sulfate: 0.5, Potassium dihydrogen phosphate: 1.5, Sodium dihydrogen phosphate: 1.5, Sodium citrate: 0.4, Magnesium sulfate: 0.025, Calcium chloride: 0.5mg, Zinc sulfate: 0.001, Copper sulfate: 0.001, L-Glutamine sodium sulfate: 0.5, Iron ammonium citrate: 0.04, Pyridoxine hydrochloride: 0.001, Biotin: 0.5mg, Malachite green: 0.25mg, Agar: 15.0, pH: 6.4-6.8 (25 ℃)

Growth conditions at 37 ℃; 3-7 days; Aerobic;

Storage conditions 2-8 ℃

Security Level 2

The morphology of the colony is 0.5-1mm in diameter, circular, with neat edges, opaque, protruding in the middle, rough surface, wrinkled surface, dry texture, G+(blue purple), rod-shaped, purity: pure

Separation of substrates for human isolation

Model strain no

Step 1: Prepare the two tablets mentioned above; ② After disinfecting the surface of the ampoule tube, open it in a safety cabinet, burn the top with an alcohol lamp, quickly drip sterile water to break it, and then use tweezers to crush it; ③ Take 0.5mL of sterile water and transfer it into a freeze-drying tube. After fully dissolving the bacterial powder, transfer it into 2 plates at a rate of 200 μ L per plate and apply evenly; ④ Place the petri dish under the above cultivation conditions for cultivation, and once the bacterial strain grows, it can be used.

Notes:

1. Microbial strains should be stored in a low temperature, clean, and dry place. Prolonged storage at room temperature can cause strain decay;

2.Intracellular mycobacteriaThe operation should be carried out under sterile conditions, and after inoculation, it should be sterilized before disposal;

3. The storage time for inclined and puncture strains is usually 3-6 months, and they should be transferred in a timely manner according to the condition of the strains; The storage time of freeze-dried powder is usually 2-25 years; The storage time of glycerol bacteria is 2-5 years.

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