Human GRO β detection kit (enzyme-linked immunosorbent assay)

personGROBTest kitEnzyme linked immunosorbent assaylaw）

【Product Name】

Common name:personGROBDetection kit (enzyme-linked immunosorbent assay)law）

English name:Human GROBAssay Kit

【specification】

96T

【intended use】

Human GRO β detection kit (enzyme-linked immunosorbent assay)Can be used for quantitative detectionpersonSerumEDTA-In plasma and cell supernatantGROBRestructuring and Natural FormsGROBAll can be detected. This product is only used for research and not for diagnosis.

[Introduction]

personGROβ is a7.9 kDaThe protein contains73One amino acid residue. humanGROβ isC-X-CA member of the chemokine family that promotes neutrophil and basophil chemotaxis and degranulation.GROSimilar to βGROAlpha specifically inhibits the proliferation of capillary endothelial cells stimulated by growth factors in a dose-dependent manner.

【Detection Principle】

This test kit is a diagnostic tool for testingThe use of human GRO βProfessional ELISA kitBased on the principle of solid-phase sandwich method, two highly specific antibodies are used for detection. Tetramethylbenzidine（TMB）As a chromogenic reagent, the color intensity is related topersonGROThe amount of β is directly proportional.

【Kit components】

1）Microplate: 96Kong, wrapped in anti human coatingGROB (C）RabbitIgGAffinity purification

2）Labeling antibodies: 30Double concentration,1x0.4ml，HRP-Anti humanGROβ RabbitIgG Fab’Affinity purification

3）Standard product: 2x0.5mlThe ReorganizerGROB

4）EIABuffer solution: 1x30ml

5）Labeling antibody solution: 1x12ml

6）Substrate solution: 1x15ml

7）Termination solution: 1x12ml

8）Wash buffer concentrate: 1x50ml，40Double concentration

【Preparation of reagents】

（1）Wash buffer solution

Balance to room temperature before use and gently mix evenly

50mLWash buffer concentrate+1950mLDistilled water is mixed evenly to obtain the washing buffer working solution. The diluted washing buffer solution can be stored in the refrigerator2week

（2）Labeling antibodies

Labeling antibody solution in disposable test tubes30Diluted labeled antibody concentrate, if used1Micro porous plate（8Hole), needed800MLLabeling antibodies, i.e30MLLabel antibody concentrate+870MLLabel antibody solution and mix evenly

Preparation should only be carried out before use, and the labeled antibody concentrate should be prepared before use4Sealed storage at ℃

（3）Standard product

Add to standard samples0.5mLDistilled water is used to fully dissolve it, resulting in a standard concentration of5000pg/mL.

prepare9Add test tubes and add them to each tube230ML EIAbuffer solution

Translate into English230MLAdd the reconstructed standard into the test tube1Gently mix well; Translate into English230MLtest tube1Add the medium solution into the test tube2Mix evenly. continuous2Double dilution preparation9The concentration of a standard substance is within the range of2500 - 19.53pg/mL, test tube8The concentration is0pg/mL, as a sample blank

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Shenzhen Kerunda Biotechnology Co., Ltd