Human GRO α/MGSA detection kit (enzyme-linked immunosorbent assay)

personGROalpha/ MGSATest kitEnzyme linked immunosorbent assaylaw）

【Product Name】

Common name:personGROalpha/ MGSADetection kit (enzyme-linked immunosorbent assay)law）

English name:Human GROalpha/MGSA Assay Kit

【specification】

96T

【intended use】

This kit can be used for quantitative detectionpersonSerumEDTA-In plasma and cell supernatantGROalpha/ MGSARestructuring and Natural FormsGROalpha/ MGSAAll can be detected. This product is only used for research and not for diagnosis.

[Introduction]

GROalpha/ MGSAasCXCSubfamily, related to amino acid sequencesIL-8Has high homology. According to reports, it also has a similar structure in three-dimensional structural analysis, andIL-8The level of leukocyte chemotaxis is almost the same. It has been reported that it is produced by activated monocytes, fibroblasts, epithelial cells, and endothelial cells. In the Helicobacter pylori gastric mucosal infection group, patients with gastric gastritis and ulcersIL-8andGROThe level of alpha was significantly higher in the non infected group.GROAlpha has been identified as a factor that promotes the proliferation of melanoma cells and is a type of cytokine with alpha, beta, and gamma functions3Polypeptides of different subtypes.GROAlpha inhibits the proliferation of endothelial cells. Due to the large number of tumor blood vessels in renal cancer, it is crucial to identify angiogenic factorsGROAlpha is particularly important in the process of angiogenesis.

【Detection Principle】

This kit is based on the principle of solid-phase sandwich method and uses two highly specific antibodies for detection. Tetramethylbenzidine（TMB）As a chromogenic reagent, the color intensity is related topersonGROalpha/ MGSAThe quantity is directly proportional.

【Kit components】

1）Microplate: 96Kong, wrapped in anti human coatingGROalpha/ MGSArabbitIgGAffinity purification

2）Labeling antibodies: 30Double concentration,1x0.4ml，HRP-Anti humanGROalpha/ MGSArabbitIgG Fab’Affinity purification

3）Standard product: 2x0.5mlThe ReorganizerGROalpha/ MGSA

4）EIABuffer solution: 1x30ml

5）Labeling antibody solution: 1x12ml

6）Substrate solution: 1x15ml

7）Termination solution: 1x12ml

8）Wash buffer concentrate: 1x50ml，40Double concentration