Fiber optic oxygen pH and temperature measuring instrument FireSting PRO

Fiber optic oxygen pH and temperature measuring instrument FireSting PRO

The revolutionary technology for measuring oxygen, pH, and temperature is particularly suitable for measuring photosynthetic oxygen release, water and soil microecology research

Fiber optic oxygen pH and temperature measuring instrument FireSting PRO main functions

The fiber optic oxygen, pH, and temperature measuring instrument adopts REDFLASH dye luminescence technology, which has the characteristics of high accuracy, high stability, low power consumption, low interference level, and fast response. It has been widely used and has scientific research achievements; And it can connect various types of optical sensors to meet different experimental needs. PyroScience has multiple new technologies and constantly strives for excellence, making the fiber optic multi analyte measuring instrument FireSting PRO a leader in high-precision oxygen measurementDirection technology improvementThe optimized design of the production process also enables FireSting PRO to maintain high product performance while maintaining a competitive price.

application field

l Measurement of dissolved oxygen in water, algae and algal biofilm, and photosynthesis and respiration of plants

lRespiratory metabolism measurement of aquatic animals (invertebrates such as fish and aquatic insects, planktonic animals)

lRespiratory metabolism measurement of terrestrial animals, experimental animals, animal tissues, blood, etc

lOxygen measurement of soil, wetland, marine sediment, river and lake sediment profiles

lOxygen measurement in bioreactors, fermentation processes, enzyme kinetics, cell culture, etc

lOxygen measurement for grain and food storage and transportation, wine, etc

lOxygen measurement for sewage treatment, biogas, landfill sites, organic matter degradation, etc

Product Features

Using REDFLASH new technology to accurately and sensitively detect oxygen, pH, and temperature

Built in humidity and atmospheric pressure sensors for fast, accurate, and uninterrupted data compensation

Measuring multiple analytes in various environments such as gas phase, liquid phase, and semi-solid phase, especially suitable for photosynthetic oxygen evolution and pH measurement

Provide 1, 2, or 4 channel versions, with one device capable of synchronously measuring oxygen, pH, and temperature

It can be connected to micro sensors, small sensors, sturdy probes, replaceable cap probes, nano probes, solvent resistant probes, as well as non-contact point optical sensors (measuring cups, breathing bottles, flow chamber chips)

Sensor end diameter ranges from 50 µ m to 3 mm

A FireSting PRO can simultaneously connect several different measurement ranges (standard and trace) and different types of analyte sensors for use together

Connect to PC via USB for power supply and control; Or independently powered, operating in Broadcast mode and simulating channel output data

Can provide OEM customization

The new version of PyroScience Workbench can connect up to 10 measuring instruments simultaneously, and can also perform drift compensation and oxygen rate calculation

Revolutionary REDFLASH technology

The REDFLASH technology invented by PyroScience is based on REDFLASH dye luminescent materials that are only sensitive to analytes. REDFLASH dye will emit near-infrared light (760nm NIR) of different intensities depending on the oxygen content when excited by red light (λ=620 nm). The measurement of NIR intensity can reflect the oxygen content. REDFLASH technology has the characteristics of high precision, high stability, low power consumption, low interference level, and fast response. Red excitation light can effectively reduce the interference of spontaneous fluorescence and decrease the stress on organisms.

REDFLASH dye emits NIR when excited by red light, and the NIR emitted gradually changes with the concentration of the analyte

Left image: A) High NIR emission under low oxygen concentration; B) Low NIR emission under high oxygen concentration

Figure: A) Low NIR emission at high pH; B) High NIR emission at low pH concentration

Main technical parameters

System composition:

FireSting-PROIt is a high-precision, compact, and based onPCofFiber optic multi analyte measuring instrumentIt can provide single channel, dual channel, and four channel versions. It can be widely used for short-term investigations or long-term monitoring in laboratories and fields, as well as for oxygen in industrial fieldspHmonitor. The host passes throughUSBInterface connection for computer power supply and control; Each model of host includes a temperature sensor interface for connectionPT100type(For example)TDIP15）Temperature probe.

1.FireSting PRO Host

The picture shows the four channel FireSting O2 The host has four sensor interfaces S1-S4, which are connected to the sensors through optical fibers; T is the TDIP15 temperature sensor interface, connected to a computer via USB.

2. Different types of sensors

*Including micro and small sensors

3. Manual Micromanigator

When precise measurement of oxygen concentration in semi-solid materials (such as sediment, biofilm, etc.) is required, especially when precise layer by layer measurement is needed, the manual micro manipulator MM33 can be used to manipulate the fiber optic oxygen sensor. MM33 can be manually positioned on X, Y, and Z axes, and the positions of the three control knobs on the three axes are very compact and convenient. After fixing the needle probe, the micro manipulator is manually controlled to accurately move the probe to the designated position inside the sample, and the operation is precise and smooth to obtain accurate measurement results. Technical parameters: Weight 650g, size 65 x 200 x 160 mm, can hold sensors with a diameter of 6.5-12mm, x/y/z axis adjustment range of 20 mm/25 mm/37 mm, x/y/z axis resolution of approximately 100 µ m/100 µ m/10 µ m.

In order to obtain stable measurements, a heavy-duty bracket HS1 or a lightweight bracket LS1 is required to stably support the MM33 micro control platform. The heavy-duty bracket for the micro control console weighs 10kg, while the lightweight bracket weighs 4.5kg. The dimensions are 400 x 360 x 560 mm, providing stable support for the micro control console; Swinging free triangular basis; Up to 4 micro control consoles can be loaded; Two installation rods with a diameter of 12mm and a height of 500mm, resistant to acid and seawater.

Application Examples

1. Determination of oxygen content in leaf tissue

图片来源： Courtesy of J. Kirchberg and M. Fischer, Martin-Luther-University, Halle-Wittenberg, and J. Bravidor, Department of Lake Research, Helmholtz Centre for Environmental Research UFZ, Magdeburg (both in Germany)

Determination of oxygen content in needle fissures

Chamaecyparis obtuse var. formosana of Taiwan, China, Taiwan, China, in the cloud forest of Taiwan, China, China, found that 90% of its pores were gathered in the cracks of the needles through microstructure investigation, and according to oxygen measurement and fluorescent tracer, it was found that water drops added to the cracks would not enter the cracks. It shows that Taiwan, China Platycladus orientalis has the characteristics of "xerophytic", such as hydrophobic, stomatal recess, stomatal aggregation, etc. There is an air layer (leaf air film) in the fissure, which can prevent sedimentation, fog and water vapor condensation from covering the stomata, so that it can maintain carbon dioxide absorption and photocooperation in the high humidity environment.

Oxygen sensor (50 μ m diameter needle tip) placed at the crack

a. Dry leaves; b. C. Add one drop of 5 μ l water droplet; d. Add three drops of 5 μ l water droplets

References:

Pariyar S , Chang S C , Zinsmeister D , et al. Xeromorphic traits help to maintain photosynthesis in the perhumid climate of a Taiwanese cloud forest[J]. Oecologia, 2017, 184(3):1-13.

Research on the Total Primary Productivity of Coral

Montastria curva, a large coral in the Great Barrier Reef of Australia, was exposed to different intensities of light in a circulation chamber, and its O value was measured with different water depths2The content was measured and the primary productivity GPP was calculated, and its quantum yield (QEs) was determined; Discovered at 640 μ mol m-2s-1Under moderate light intensity, although the energy utilization efficiency is very low (only 4%), it achieves a high photosynthetic efficiency (0.1 O)2photon-1）. Coral is an efficient light collector that can distribute light on the coral/tissue microstructure canopy, resulting in a high quantum yield of photosynthetic microalgae.

Photosynthetic rate under intensity (O)2Determination of Content Calculation

Experimental setup (a) and oxygen sensor layout (b)

Under four different light intensities (160, 320, 640, and 1280 μ mol/m)-2s-1）Total primary productivity (nmol O)2cm-3s-1）(Black bar chart) and corresponding O2Vertical distribution map of water depth with concentration (μ M) (lines and dots)

References

Brodersen K , Lichtenberg M , Ralph P , et al. Radiative energy budget reveals high photosynthetic efficiency in symbiont-bearing corals[J]. Journal of the Royal Society Interface, 2014, 11(93):20130997.

Determination of oxygen content in coastal underwater meadows

In situ monitoring of oxygen in seagrass meadows in the coastal waters of Byala Bay (A) in Bulgaria and Hoopo Bay (B) in South Korea, and calculation of oxygen flux using vorticity covariance technique. The average daily net oxygen fluxes of BY and HP coastal seagrass meadows are -474 to 326 mmol O, respectively2m-2d-1And -74 to 482 mmol of O2m-2d-1And net O2There is a significant correlation between yield and photosynthetically active radiation (PAR) (P-I curve); Flow rate between 3.30 and 6.70 cm/s-1At that time, community metabolism significantly increased by 20 and 5 times during the day and night, respectively, indicating that flow velocity may be the main influencing factor of community photosynthesis. Ultimately, the net ecosystem metabolism of BY is -17mmol O2m-2d-1, for heterotrophic; And HP is 36mmol O2m-2d-1It is in a self-sustaining state.

Oxygen measurement of seagrass in the coastal waters of Byala Bay (A) in Bulgaria and Hoopo Bay (B) in South Korea

15 minute oxygen flux and photosynthetically active radiation (PAR) of seagrass in the coastal waters of BY Bay (upper) and HP Bay (lower); Positive flux represents the release of O from seagrass2

References

Lee J S , Kang D J , Hineva E , et al. Estimation of net ecosystem metabolism of seagrass meadows in the coastal waters of the East Sea and Black Sea using the noninvasive eddy covariance technique[J]. Ocean Science Journal, 2017, 52(1):1-14.

In situ determination of oxygen content in deep-sea sediments

In situ detection of oxygen flux in deep-sea sediments (2500m) in the Arctic was conducted. Under the constraints of low oxygen gradient and flux, low turbulence, and low particle content in the sediment boundary layer, the vorticity covariance turbulent oxygen flux (-0.9+-0.2 (SD) mmol O) was calculated2m-2d-1）Dissolved oxygen flux (-1.02+-0.3 (SD) mmol O)2m-2d-1）Total oxygen uptake measured by sediment culture chamber method (-1.1+-0.1 (SD) mmol O)2m-2d-1）Consistent, indicating that the site is mainly mediated by microbial oxygen consumption, and the average carbon mineralization rate of benthic organisms is 4.3gC m2yr-1Exceeding the annual sedimentation amount of particulate organic matter measured by the sediment collector.

Image source:Dr. Frank Wenzhoefer, group for Deep Sea Ecology and Technology, Alfred-Wegener-Institute for Polar and Marine Research, Bremerhaven, and Max-Planck-Institute for Marine Microbiology, Bremen (both inGermany)

References

Donis D , Mcginnis D F , Holtappels M , et al. Assessing benthic oxygen fluxes in oligotrophic deep sea sediments (HAUSGARTEN observatory)[J]. Deep-Sea Research Part I, 2016, 111:1-10.

Determination of Organizational Support for 6 European Sea Bass

The lake in Forsmark, Sweden, has experienced a slow increase in water temperature due to the extraction of cold water for cooling purposes by the nuclear power plant, and the direct injection of warm sewage from the surrounding area. Many species of fish have disappeared, with European sea bass becoming the dominant species. The aerobic metabolic rate and venous oxygen partial pressure of sea bass (Perca fluviatilis Linnaeus) under high temperature were measured using an optical oxygen sensor, and the critical high temperature CTmax was determined. It was found that the oxygenation operation caused a double increase in the aerobic range at 23 ° C, while the critical high temperature CTmax (34.6 ± 0.1) did not significantly increase compared to the control (34.0 ± 0.5 ° C); However, there was no significant difference in aerobic range and CTmax between hypoxic operation and control. Oxygen limitation is not a universal mechanism determining CTmax in fish.

Implanting a customized optical sensor into the venous sinus for continuous measurement of oxygen partial pressure in venous blood

Image source:Timothy Clark – Australian Institute of Marine Science, Townsville, Australia. （左） Erik Sandblom – University of Gothenburg, Göteborg, Sweden. （右）

Metabolic reactions of European sea bass under oxygen enriched hyperoxic and anaerobic anaemic conditions

A. Oxygen consumption rate (M ̇ O)2）Not affected by processing; B. 23 ° C Standard Metabolic Rate (SMR), Maximum Metabolic Rate (MMR), and Aerobic Range (AS)

References

Brijs J , Jutfelt F , Clark T D , et al. Experimental manipulations of tissue oxygen supply do not affect warming tolerance of European perch[J]. Journal of Experimental Biology, 2015, 218(15):2448-2454.

Determination of oxygen in artificial soil columns where earthworms live

After adding compacted soil blocks prepared from glucose into an artificial soil column, changes in oxygen content in the column were detected, and it was found that the oxygen content decreased over time. This is because the addition of glucose enhances the biological respiration of soil animals (earthworms), consuming oxygen and producing carbon dioxide.

Monitoring oxygen changes in soil columns using oxygen sensors

After adding glucose soil blocks to the soil column, the soil oxygen content (%) decreases over time

Image source:Hochschule Osnabrück (Germany), Faculty of Agricultural Sciences and Landscape Architecture

Origin: PyroScience, Germany