Fiber optic oxygen measuring instrument FireSting-O2

Fiber optic oxygen measuring instrumentFireSting-O2

The revolutionary technology of oxygen measurement is particularly suitable for measuring photosynthetic oxygen release, water and soil microecology research

Main functions:

Fiber optic oxygen measuring instrument adoptsRedflashDye luminescence technology has the characteristics of high precision, high stability, low power consumption, low interference level, and fast response, and has been widely applied and researched; And it can connect various types of oxygen sensors to meet different experimental needs.PyroScienceHaving multiple new technologies and constantly striving for excellence, the fiber optic oxygen measuring instrumentFireSting-O2Becoming a pioneer in the field of high-precision oxygen measurementRecommended companiesOptimizing the production process of design also enablesFireSting-O2Maintain competitive prices while maintaining high product performance.

Fiber optic oxygen measuring instrument FireSting-O2Application fields:

l Measurement of dissolved oxygen in water, algae and algal biofilm, and photosynthesis and respiration of plants

l Respiratory metabolism measurement of aquatic animals (invertebrates such as fish and aquatic insects, planktonic animals)

l Respiratory metabolism measurement of terrestrial animals, experimental animals, animal tissues, blood, etc

l Oxygen measurement of soil, wetland, marine sediment, river and lake sediment profiles

l Oxygen measurement in bioreactors, fermentation processes, enzyme kinetics, cell culture, etc

l Oxygen measurement for grain and food storage and transportation, wine, etc

l Oxygen measurement for sewage treatment, biogas, landfill sites, organic matter degradation, etc

Product Features:

l adoptRedflashNew technology for precise and sensitive detection of oxygen

l Built in humidity and atmospheric pressure sensors for fast, accurate, and uninterrupted data compensation

l Measuring oxygen content in various environments such as gas phase, liquid phase, and semi-solid phase is particularly suitable for measuring photosynthetic oxygen evolution

l provide1，2or4Channel version

l It can be connected to micro sensors, small sensors, sturdy probes, replaceable cap probes, nano probes, solvent resistant probes, as well as non-contact point sensors (measuring cups, breathing bottles, flow chamber chips)

l Sensor end diameter from50 µmto3 mm

l aFireSting-O2Can simultaneously connect several different measurement ranges (standard and trace) and different types of analyte sensors for use together

l throughUSBconnectionPCPower supply and control; Or independently powered, usingBroadcastMode operation and simulation channel output data

l can provideOEM定制

l new versionPyroScience WorkbenchCan be connected simultaneously10An oxygen measuring instrument can also be used forDrift compensation and oxygen rate calculation

revolutionaryRedflashtechnology

fromPyroScienceinventedRedflashTechnology based on sensitivity only to oxygenRedflashDye luminescent materials.RedflashDyes are affected by red light(λ=620 nm)After excitation, near-infrared light of different intensities will be emitted according to the oxygen content（760nm NIR）. throughNIRThe measurement of intensity can reflect the oxygen content.RedflashTechnology has the characteristics of high precision, high stability, low power consumption, low interference level, and fast response. Red excitation light can effectively reduce the interference of spontaneous fluorescence and decrease the stress on organisms.

RedflashWhen the dye is excited by red light, it will emitNIREmitted as the oxygen concentration increasesNIRGradually decreasing (quenching effect).A）High under low oxygen concentrationNIRLaunch;B）Low under high oxygen concentrationNIRlaunch

Main technical parameters:

System composition:

FireSting-O2It is a high-precision, compact, and based onPCThe fiber optic oxygen measuring instrument is available in single channel, dual channel, and four channel versions. It can be widely used for laboratory and field short-term investigations or long-term monitoring, as well as oxygen monitoring in the industrial field. The host passes throughUSBInterface connection for computer power supply and control; Each model of host includes a temperature sensor interface for connectionPT100Type (e.gTDIP15）Temperature probe.

1. FireSting-O2host

2. Different types of sensors

3. manualMicro control console（Micromanipulator）

When precise measurement of semi-solid materials (such as sedimentbiofilmWhen measuring the oxygen concentration, especially when precise stratification is required, a manual micro controller can be usedMM33Manipulate fiber optic oxygen sensors.MM33canX，Y，ZAxis manual positioning, the positions of the three control knobs for the three axes are very compact and convenient. After fixing the needle probe, the micro manipulator is manually controlled to accurately move the probe to the designated position inside the sample, and the operation is precise and smooth to obtain accurate measurement results.Technical parameters:heavy650g, size65 x 200 x 160 mm, can be fixed6.5 - 12mmDiameter sensors,x / y / zAxis adjustment range20 mm / 25 mm / 37 mm，x / y / zAxis resolution is approximately100 µm / 100 µm / 10 µm.

In order to obtain stable measurements, it is necessary toheavy-duty bracketHS1Or lightweight bracketLS1To provide stable supportMM33Micro control console. Heavy duty bracket for micro control console10kgLightweight bracket4.5kg; Size400 x 360 x 560 mmStable support micro control platform; Swinging free triangular basis; Maximum load capacity4Micro control console;2root diameter12mm, high500 mmThe installation rod is resistant to acid and seawater.

Application example:

1.Determination of oxygen content in leaf tissue

2.Determination of oxygen content in needle fissures

Taiwan, China Platycladus orientalis in the cloud forest of Taiwan, China, ChinaChamaecyparis obtuse var. formosanaThrough microstructural investigation, it was found that90%The pores of the needle are concentrated in the cracks, and according to oxygen measurement and fluorescent tracing, it was found that water droplets added to the cracks do not enter the cracks. It indicates that Taiwan, China Platycladus orientalis has hydrophobicity, stomatal recess, stomatal aggregation, etc“Drought tolerant type”The characteristic is that there is an air layer (leaf air film) in the cracks, which can prevent sedimentation, fog, and water vapor condensation from covering the stomata, allowing them to maintain carbon dioxide absorption and photosynthesis in high humidity environments.

oxygen sensor(50μmDiameter needle tip)Place it in the crack.a.Dry leaves;b, c.Add one drop5μlWater droplets;d.Add three drops5μlwater droplet

References：Pariyar S , Chang S C , Zinsmeister D , et al. Xeromorphic traits help to maintain photosynthesis in the perhumid climate of a Taiwanese cloud forest[J]. Oecologia, 2017, 184(3):1-13.

3.Research on the Total Primary Productivity of Coral

Large coral reefs in the Great Barrier Reef of AustraliaShort hikeIlluminate different intensities of light in the circulation chamber and measure it with different depths of waterO2Content and calculation of primary productivityGPPAnd its quantum yield was measured(QES)Discovered in640 μmol m-2 s-1Under moderate light intensity, although the energy utilization efficiency is very low (only4%）But achieving high photosynthetic efficiency（0.1 O2photon-1）. Coral is an efficient light collector that can distribute light across the coral/Organize microstructure on the canopy layer to achieve high quantum yield of photosynthetic microalgae.

The photosynthetic rate of corals under different light intensities（O2Experimental setup for content calculation and determination（a）Arrangement of oxygen sensors（b）

Under four different light intensities(160、320、640and1280 μmol m-2s-1)Total primary productivity(nmol O2cm-3s-1) (Black bar chart)And correspondingO2concentration(μM)(Lines and dots)Vertical distribution map of water depth

References:Brodersen K , Lichtenberg M , Ralph P , et al. Radiative energy budget reveals high photosynthetic efficiency in symbiont-bearing corals[J]. Journal of the Royal Society Interface, 2014, 11(93):20130997.

4.Determination of Oxygen Content in Coastal Submarine Meadows

In situ monitoring in BulgariaByalaGulf（A）And KoreaHoopoGulf（B）Oxygen flux of seagrass meadows in coastal waters is calculated using vorticity covariance technique.BYandHPThe average daily net oxygen flux of two coastal seagrass meadows is:-474to326mmol O2m-2d-1and-74to482 mmol O2m-2d-1And netO2Yield and photosynthetically active radiation(PAR)Show significant correlation(P-Icurve)The flow rate is3.30 to6.70 cm s-1At that time, community metabolism significantly increased during the day and at night, respectively20and5This indicates that flow velocity may be the main influencing factor of community photosynthesis. Finally,BYThe net ecosystem metabolism is-17 mmol O2m-2d-1, for heterotrophic; ButHPfor36mmol O2m-2d-1It is in a self-sustaining state.

BulgariaByalaGulf（A）And KoreaHoopoGulf（B）Oxygen measurement of seagrass in coastal waters

BYGulf (Above) andHPSeaweed in the coastal waters of the Gulf (Lower)15Minute oxygen flux and photosynthetically active radiation(PAR)Positive flux represents the release of seagrassO2

References：Lee J S , Kang D J , Hineva E , et al. Estimation of net ecosystem metabolism of seagrass meadows in the coastal waters of the East Sea and Black Sea using the noninvasive eddy covariance technique[J]. Ocean Science Journal, 2017, 52(1):1-14.

5.In situ determination of oxygen content in deep-sea sediments

In situ detection of the deep sea in the Arctic（2500m）The oxygen flux in sediment is calculated based on the vorticity covariance turbulent oxygen flux under the constraints of low oxygen gradient and flux, low turbulence, and low particle content in the sediment boundary layer(-0.9+-0.2 (SD) mmol O2m-2d-1)Dissolved oxygen flux(-1.02+-0.3 (SD) mmol O2m-2d-1)Total oxygen uptake measured by sediment culture chamber method(-1.1+-0.1(SD) mmol O2m-2d-1)Consistent, indicating that this site is mainly mediated by microbial oxygen consumption, and the average carbon mineralization rate of benthic organisms is4.3gC m2yr-1Exceeding the annual sedimentation amount of particulate organic matter measured by the sediment collector.

Image source: Dr. Frank Wenzhoefer,groupfor Deep SeaEcologyandTechnology, Alfred-Wegener-InstituteforPolarandMarine Research, Bremerhaven, and Max-Planck-InstituteforMarineMicrobiology, Bremen (both inGermany)

References：Donis D , Mcginnis D F , Holtappels M , et al. Assessing benthic oxygen fluxes in oligotrophic deep sea sediments (HAUSGARTEN observatory)[J]. Deep-Sea Research Part I, 2016, 111:1-10.

6.Determination of tissue oxygen supply in European sea bass

SwedenForsmarkDue to the cooling of the nuclear power plant, cold water was pumped out of the lagoon, and warm sewage from the surrounding area was directly injected, causing a slow increase in water temperature. Many types of fish have disappeared, and European sea bass has become the dominant species. Measurement of Sea Bass at High Temperature Using Optical Oxygen Sensor(Linneaus fluviatilian path)Measure the aerobic metabolic rate and venous oxygen partial pressure, and determine the critical high temperatureCTmaxIt was found that the oxygenation operation caused23°CDouble the aerobic range and increase the critical high temperatureCTmax(34.6±0.1)Compared to the control（34.0±0.5°C）There was no significant increase; And hypoxic operation operates within the aerobic range andCTmaxThere was no significant difference between the aspect and the control group. Oxygen limitation does not determine fish speciesCTmaxThe universal mechanism.

Implant a customized optical sensor into the venous sinus for continuous measurement of oxygen partial pressure in venous blood.

Image source: Timothy Clark– Australian Institute of Marine Science, Townsville, Australia.(Left)Erik Sandblom– University of Gothenburg, Göteborg, Sweden.(Right)

European sea bass in enriched oxygenhyperoxicAnd hypoxiaanaemicThe metabolic reactions below.A.Oxygen consumption rate(ṀO2)Not affected by processing; B. 23°CStandard metabolic rate(SMR),maximum

metabolic rate(MMR)And aerobic range(AS)

References：Brijs J , Jutfelt F , Clark T D , et al. Experimental manipulations of tissue oxygen supply do not affect warming tolerance of European perch[J]. Journal of Experimental Biology, 2015, 218(15):2448-2454.

7.Determination of Oxygen in Artificial Soil Columns for Earthworm Life

After adding compacted soil blocks prepared from glucose into an artificial soil column, changes in oxygen content in the column were detected, and it was found that the oxygen content decreased over time. This is because the addition of glucose enhances the biological respiration of soil animals (earthworms), consuming oxygen and producing carbon dioxide.

Monitoring oxygen changes in soil columns using oxygen sensors

After adding glucose soil blocks to the soil column, the soil oxygen content（%）Decrease over time

Image source:Hochschule Osnabrück (Germany), Faculty of Agricultural Sciences and Landscape Architecture

Origin: GermanyPyro Science