Escherichia coli CMCC (B) 44102 (quantitative strain)

This product is a freeze-dried product containing a certain amount of microorganisms. As a quantitative quality control sample.

Main componentsThe main components are bacterial strains and freeze-drying protectants.

Source of strain: The supplier of standard strains is international and national strain collection centers, with a passage count of ≤4.

Storage conditions and expiration date-20 ℃ and below, expiration date12month

【 Usage 】

1.Flat plate coating method:

(1) Before use, balance the unopened penicillin bottle to room temperature.

(2) Aseptic operation: Open the quantitative strain bottle stopper, use a pipette to aspirate 1.1mL of the complex solution into the penicillin bottle, cover the bottle stopper, and vortex and shake5～10sMake the ball completely dissolved.

(3) Use a pipette to aspirate 100 μ mLSpread the bacterial solution evenly onto the test medium and control medium using a coating rod.

(4) Invert the plate and cultivate it under suitable conditions.

2. Liquid inoculation or pouring dish method:

(1) Before use, balance the unopened penicillin bottle to room temperature.

(2) Aseptic operation: Open the quantitative strain bottle stopper, use a pipette to aspirate 1.1mL of the complex solution into the penicillin bottle, cover the bottle stopper, and vortex and shake5～10sMake the ball completely dissolved.

(3) Use a pipette to aspirate1mLMicrobial solution, add9mLMix well in sterile saline solution.

(4) Suck the above bacterial solution, add it to the test liquid culture medium and counting or reference culture medium, and incubate under suitable conditions. Or add it to a sterile empty petri dish, inject 15mL to 20mL of melted test medium with a temperature not exceeding 46 ℃, mix well, and cover with 5mL of medium after the medium solidifies,Invert and culture after solidification.

Escherichia coli CMCC (B) 44102 (quantitative strain)Appraisal result:

1. Characteristics of bacterial colonies on EMB agar plates and Escherichia coli agar plates:

EMB plate: It is a round moist colony with a metallic luster and black center, as shown in Figure 1.

Escherichia coli color plate: showing blue-green colonies, as shown in Figure 2.

Figure 1 Colony characteristics on EMB plate	Figure 2 Colony characteristics on Escherichia coli color plate

2. Gram staining microscopy: Gram negative bacteria.

Figure 3 Gram staining microscopy

3. Biochemical testing of Gram negative bacteria identification system by Bojian: Escherichia coli.

Figure 4 Biochemical detection results of Bojian Gram negative bacteria identification system

【Escherichia coli CMCC (B) 44102 (quantitative strain)Product performance indicators

This product is a freeze-dried water-soluble ball containing a certain amount of microorganisms. The ball is a white approximately spherical shape. After cultivation, there should be no growth of miscellaneous bacteria.

Since its establishment, Haibo Biological Company has been dedicated to the research and production of microbial culture media. Its products include microbial dry powder culture media, particle culture media, colorimetric culture media, ready to use culture media, animal vaccine culture media, plant tissue culture media, cell culture media, and various matching reagents, with over 1000 varieties. The product is strictly produced and quality controlled in accordance with domestic and foreign standards such as GB standards, SN standards, Chinese Pharmacopoeia standards, WS standards, YY/T standards, ISO standards, FDA standards, Japanese standards, USP standards, EP standards, BP standards, etc.

The company is equipped with a well-equipped laboratory and standardized factory, with numerous technical personnel, a mature R&D team, and a strict quality monitoring system to ensure the technical excellence and quality stability of the culture medium.

Current products of Haibo Biotechnology Company
Dry powder culture medium, animal vaccine culture medium, pellet culture medium, chromogenic culture medium, ready to use sterile booster culture medium, ready to use tubular culture medium, ready to use cloud delivery culture medium, disposable plate, HBI microbial biochemical identification strip, Bo Jian Gram negative bacterial identification system, sterile homogenization bag/sampling bag, porcelain bead bacterial preservation tube, HBTube high transparency test tube.

Haibo Biotechnology Co., Ltd. aspires to become a large-scale high-tech enterprise that integrates biological genetic engineering, biopharmaceuticals, and biological reagents. At present, we are continuing to apply for high-tech projects in Qingdao, Shandong, and the country, striving for government support to lay a solid foundation for the company's development.
In the further development of the company, various talents and more excellent technologies are needed. Haibo Company warmly welcomes ambitious individuals to join our collective and cooperate with Haibo in various forms to become a part of the company and enjoy success in the journey ahead