Cytek Northern Lights flow cytometer

Cytek Northern Lights flow cytometerSupports single, dual, and triple laser configurations, equipped with multiple flow bottles and level sensors, enabling high-precision sample analysis. Absolute counting is achieved through volumetric sampling mode, supporting fast and high-throughput injection, providing multi-channel fluorescence detection and high-resolution scattered light detection. The workstation is compatible with different laser configurations, the computer system runs Windows 10 Pro, and the monitor is 32 "UHD4K. The liquid flow rate is adjustable and supports multiple types of sample tubes. The environment requires strict control of temperature, humidity, and air quality.

Cytek Northern Lights flow cytometerTechnical Specifications:

Optical Path: Excitation Optical Path

Standard liquid flow bottle: a 4L liquid flow bottle containing a liquid level sensor, compatible with 20L sheath liquid bottles and waste liquid bottles.

The pulse shape parameters include pulse area and pulse height, the scattered light channel includes pulse width, and only one fluorescence channel can select pulse width under each laser.

Absolute counting can accurately calculate the concentration of each μ L sample using volumetric loading mode.

The specifications of workstations with different laser configurations may vary. Computer operating system: Windows ® 10 Pro 64 bit processor: Intel ® Core ™ I7 processor memory: 6 GB hard drive: 500GB SSD and 1 TB SATA graphics card: NVIDIA ® GeForce monitor 32 "UHD 4K monitor

Laser: Single laser configuration: 488nm: 50mW

Dual laser configuration: 488nm: 50mW, 640nm: 80mW

Three laser configurations: 405nm: 100mW, 488nm: 50mW, 640nm: 80mW

High throughput sampling device: ASL and AMS temperature control module: 4-30 ° C (AMS only)

High throughput sample analysis speed ASL: 27 min to complete 96 well plate analysis AMS: 35 min to complete 96 well plate analysis

High throughput injection method ASL: Standard 96 well plate, 96 well deep hole plate, 40 well tube holder (12X75mm) AMS: Standard 96 well plate

Cross contamination rate of orifice plate: standard mode: ≤ 0.3%; Low speed mode: ≤ 0.1%; High speed mode: ≤ 1%

Forward and lateral detection FSC: a high-performance semiconductor detector with a 488nm bandpass filter. SSC: Two high-performance semiconductor detectors with 405nm and 488nm bandpass filters. Note: 405nm side scattered light is only configured for 3 laser machines.

Performance: Fluorescent linear FITC R2 ≥ 0.995/PE R2 ≥ 0.995

Standard optical path configuration: Purple detector module: (only available in 3 laser machines); 16 channels with non-uniform spectral width configuration of 420-829nm. Blue detector module: 14 channels with non-uniform spectral width configuration of 498-829nm. Red detector module: 8 channels with non-uniform spectral width configuration of 652-829nm.

Liquid flow sample flow rate: low speed: 15 μ L/min, medium speed: 30 μ L/min, high speed: 60 μ L/min, orifice plate high-throughput mode: 100 μ L/min.

Environmental requirements: Power supply 100-140 VAC, 15A or 200-250 VAC, 10A; Heat dissipation of 500 W for all solid-state lasers; Temperature 15-28 ° C; humidity 20% -85% non condensing; Air filtration should not contain dust or smoke; There are no special requirements for lighting.

Sample tube type: 12x75mm polystyrene or polypropylene flow tube.

Cross contamination:<0.1%

Liquid flow mode: Long cleaning mode, SIT flushing mode, cleaning filter mode, cleaning flow chamber mode.

Forward and lateral resolution: RESOLUTION can detect lymphocytes, monocytes, and granulocytes.

Lateral detector resolution: capable of detecting 0.2 μ m microspheres from noise.