Aspergillus fumigatus (AF) IgM antibody kit

Aspergillus fumigatus (AF) IgM detection kit

Aspergillus fumigatusIgMDetection kit (enzyme-linked immunosorbent assay)

English name:NovaLisa®Aspergillus fumigatus IgMELISA

IntroducingAspergillus fumigatus (AF) IgM antibody kitBefore that, let's first understandAspergillus fumigatus, a type of fungus belonging to the Aspergillus genus, is one of the most common fungal species that causes diseases. Aspergillus fumigatusfungusIt is a widely existing saprophytic organism in nature, typically found in soil and decaying organic matter. Fungal colonies produce thousands of small conidia（2-3Mm）It is easily transmitted through the air. This fungus is capable of50°CGrowing at a certain temperature, conidia grow in70°CSurvive below. Humans are constantly exposed to these asexual spores, and it is estimated that one person inhales hundreds of them every day; Usually, these substances are quickly eliminated by the immune system. Aspergillus disease mainly occurs in individuals with weakened immune function, whether due to disease or immunosuppressive drugs, and is the main cause of death in acute leukemia and hematopoietic stem cell transplantation. The term aspergillosis includes many different diseases caused by fungi of the Aspergillus genus. The most common form isallergicBronchopulmonary aspergillosis（ABPA）Pulmonary aspergilloma and invasive aspergillosis（IA）. The characteristic of allergic bronchopulmonary aspergillosis is an overreaction of the immune system to Aspergillus species. It occurs in patients with asthma or cystic fibrosis. In clinical practice,ABPAManifested as bronchial asthma with transient lung infiltration, it can progress to proximal bronchiectasis and pulmonary fibrosis. Aspergillus tumors, commonly referred to as "fungal spheroids," occur in existing lung cavities caused by tuberculosis, sarcoidosis, or other bullous lung diseases. This fungus settles and grows in a hole until it forms a compact sphere containing dead tissue from surrounding lungs, mucus, and other debris. Patients are usually asymptomatic and often coexist with aspergilloma for decades before accidental diagnosis. However, it may cause hemoptysis. Invasive aspergillosis is a rapidly progressing and usually fatal disease. It is targeted at patients with severe immune dysfunction, including leukemia and other hematological malignancies, those who have received solid organ or hematopoietic stem cell transplantation, and patients with chronic granulomatous disease or advanced AIDS.IAIts characteristic is invasion of blood vessels, leading to multifocal infiltration. It may spread to other organs, especially the central nervous system.

When Aspergillus fumigatus infection occurs in the lungs, the early symptoms are not specifically different from pneumonia caused by other causes, but prolonged infection can cause hemoptysis symptoms. Once hemoptysis occurs, it represents invasion of blood vessels and the condition will progress rapidly. Therefore, the patient's epidemiological history, contact history, and travel history should be taken into account,Aspergillus fumigatus (AF) IgM antibody kitCan be used for qualitative analysisCheck if it existsAspergillus fumigatusInfected.GermanyIBLandNovaTecProduced by a diagnostic companyAspergillus fumigatusReagent products，based onELISAEnzyme linked immunosorbent assay (ELISA) technologyAfter the experiment was completed, the results>11NTU is judged as positive.

【Kit components】

1）Microporous plate 12x8，Packaging with Aspergillus fumigatus antigen

2）Sample diluent 1×100ml, phosphate buffer solution（10mM），pH 7.2±0.2, including anti humanIgG（RFAdsorbent),ready-to-use

3）Stop Solution 1×15mlSulfuric acid,0.2mol/L，ready-to-use

4）Wash concentrated solution 20Triple concentration, phosphate buffer solution（0.2M），1×50ml，pH 7.2±0.2

5）Enzyme linked compounds 1×20mlPeople labeled with peroxidaseIgMAntibodies,ready-to-use

6）Substrate solution 1×15ml，3,3’, 5,5’-Tetramethylbenzidine（TMB），ready-to-use

7）Positive quality control 1×2mlReady to use model

8）Critical Quality Control 1×3mlReady to use model

9）Negative control 1×2mlReady to use model

10）Sealing paper 1x

11）User Manual 1x

【Required materials and equipment】(Not provided)

1）Microplate Reader

2）37℃ incubator

3）Manual or automatic board washing device

4）Pipette:10-1000µL

5）Vortex mixer

6）distilled water

7）disposable test tube

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