Apolipoprotein alpha detection kit (lipid metabolism)

Apolipoprotein alpha detection kit (lipid metabolism)，ELISAFrench imported reagent kit, English name:Human ApoA5 Assay Kit - IBL

Product Specifications

96T

Expected use of the product

This productFor scientific research purposes onlyNot used for clinical diagnosis.

Detection Principle

This reagent kit is a solid-phase sandwichELISA(Enzyme linked immunosorbent assay). When packaging the first antibody onto the plate, add the sample and standard to itPerform the first reaction in the plate hole. After the reaction, addHRP-Conjugated secondary antibody entersPlate hole drillingsecondtimeReaction. After rinsing off unbound secondary antibodies, take tetramethylbenzidine（TMB）Add to the hole and color it.

detectionsample

personSerum andEDTA-plasma

Storage conditions

2-8℃preservation,The expiration date can be found on the outer packaging of the reagent kit

Ingredients of the reagent kit

1 Pre packaged board:Anti humanApoA5（53A2）Mouse monoclonal antibody 96T x 1

2 Enzyme labeled antibody:

(30Double concentration)HRPmarkresistpersonApoA5(63A2)mouseIgGFab’） 0.4mL x 1

3 reference standard:Recombinant humanApoA5 0.5mL x 2

4 EIAbuffer solution: 30mL x 1

5 Label antibody diluent: 12mL x 1

6 Chromogenic agent: 15mL x 1

7 Stop Solution: 12mL x 1

8 Concentrated washing solution: 50mL x 1

Experimental operation steps

prepare

1)Preparation of detergent

The washing solution of the reagent kit is a type40Concentrated solution,Should be diluted to1XWash the board with detergent. Before use, equilibrate to room temperature and mix well,Configure according to the required quantity.

2）Preparation of enzyme labeled antibodies

The labeled antibody in the reagent kit is a type of30Double concentrated substance,Dilute with labeled antibody diluent according to the required amount.The diluted labeled antibody can only be used for the detection step.

example:

If you use a board(8Kong),We need it800μL.The labeled antibody.use870 μLDiluted with labeled antibody diluent30 μLconcentrate,Mix thoroughly.And add each hole separately100 μLThis step can only be performed before adding enzyme markers

remainingconcentrateThe labeled antibody solution needs to be sealed in a bottle,Exist in4°C

2)Preparation of standard products

plus0.5 mLofEIAbuffer solutionTo bottled standard productsFully dissolved in the middle,manufactured40ng/mL.Standard liquid，The dissolved standard can be stored frozen, but repeated freeze-thaw cycles should be avoided.

Dilution of standard samples

prepare7Branch test tube,And add each one separately230 μL EIAbuffer solution.

Translate into English40ng/mLThe standard product230ulAdd to test tube-1Mix well and make20ng/mLThe standard product, then take it again20ng/mLThe standard product230ulTo the test tube-2Mix well,manufactured10ng/mLStandard products, and so on, do2Dilute by multiple times to make20-0.31ng/mLThe standard product series.

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