Adiponectin Metabolism Detection Kit for Adiponectin

Adiponectin Metabolism Detection Kit for AdiponectinEnzyme linked immunosorbent assay, IBL assay kit, imported assay kit, represented by Kerunda Biotech

English name:Adiponectin ELISA

96T

forQuantitative determination of human serum and plasmaofAdiponectin.

[Introduction]

Adiponectin is30kDaThe percentage of protein in serum protein is0.01%. It is mainly synthesized by adipocytes, and muscle cells and liver cells also have the ability to synthesize adiponectin. From the original adhesive sampleNEnd and sphericalCComposed of terminal structural domains. Adiponectin in the body appears together with different oligomers. In addition to trimers and dimers, there are also high molecular weight polymers. Given two different receptors, although their distribution in tissues varies, both receptors are universally expressed. Adiponectin receptor1（AdipoR1）Especially the muscle tissue synthesized in the liver andAdipoR2In the middle.Adiponectin is also involved in the inflammatory process, therefore it is of great significance for the occurrence of arteriosclerosis and coronary artery inflammation. Therefore, measuring the level of adiponectin in plasma can be used to estimate the risk of coronary heart disease. Adiponectin also affects further physiological processes, such as angiogenesis.

【 Detection Principle 】

This kit is a sandwich assay that uses two specific and highly affinity antibodies. Adiponectin and encapsulation in the samplemicroplateThe first antibody binds to it. In the following steps, the second specific anti adiponectin antibody binds to the fixed adiponectin. The second type of antibody is biotinylated and will interact with streptavidin-peroxidase-Enzyme conjugates are mixed for use. In a closed substrate reaction, the color change will be quantitatively catalyzed depending on the adiponectin level of the sample.

Composition of reagent kit

1. Microplate,96T, coated with anti human leptin rabbitIgGAntibody, affinity purification.

2. Labeling antibody concentration,30X，0.4ml，HRPmarkedAnti human leptin rabbitIgG Fabfragment.

3. Standard products,0.5ml，2Zhi, recombinant human leptin.

4. EIABuffer solution,30ml，1%ofBSA, including0.05%The Twain20ofPBS.

5. Label antibody diluent,12ml，1%ofBSA, including0.05%The Twain20ofPBS.

6. Colorants,TMB，15ml.

7. Termination liquid,1NSulfuric acid,12ml.

8. Wash buffer concentrate,40X，50ml，0.005%Twain20Phosphate buffer solution.

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