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Tryptone soy broth medium (TSB)
NegotiableUpdate on 05/06
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Overview
Tryptone soy broth medium (TSB) (soy peptone broth) is used for microbial enrichment culture. Tryptone and soy peptone provide carbon and nitrogen sources as well as other substances required for microbial growth; Sodium chloride maintains osmotic pressure.
Product Details
Product Name:Tryptone soy broth medium (TSB)
English name: Tryptone Soy Broth Medium (Soybean Pepper Broth) (TSB)
Product number and packaging specifications:
| Product Number | Product Type | Packaging specifications |
|---|---|---|
| 024047 | dry powder | 250g/bottle |
Product UsageUsed for microbial enrichment cultivation.
Inspection principle:Tryptone and soy peptone provide carbon and nitrogen sources as well as other substances required for microbial growth; Sodium chloride maintains osmotic pressure.
Pancreatic peptone soy broth (TSB) formula ingredients:
| Formula (per liter) | content |
|---|---|
| peptone | 15.0g |
| soy peptone | 5.0g |
| sodium chloride | 5.0g |
| Final pH 7.0-7.4 | |
Usage:Weigh 25.0g of dry powder of this product, add 1 L of distilled water or deionized water, stir, heat and boil until completely dissolved, and sterilize at 121 ℃ for 15 minutes under high pressure.
Quality control（The following quality control strains were inoculated into the test medium, and the results after 24 hours are as follows:）
| name | indicator | Quality control strain and number | culture conditions | Counting medium | method | standard value | Characteristic response |
|---|---|---|---|---|---|---|---|
| Tryptone soy broth medium (TSB) | growth rate | Escherichia coli 8099 | 36±1℃，18-24h | TSA | 半定量 | Turbidity 2 | Cloudy broth |
| Staphylococcus aureus ATCC 6538 | Turbidity 2 | Cloudy broth | |||||
| Klebsiella pneumoniae CMCC (B) 46117 | Turbidity 2 | Cloudy broth | |||||
| Bacillus cereus CMCC (B) 63301 | Turbidity 2 | Cloudy broth | |||||
| Bacillus subtilis var. niger ATCC9372 | Turbidity 2 | Cloudy broth |
Storage conditions and shelf life:Store in a dark and dry place, with an unopened shelf life of three years.
References:
1. GB/T 15981-2021 Evaluation Method for Sterilization Effect of Disinfection Instruments
2. GB/T 20944.2-2007 Evaluation of Antibacterial Properties of Textiles Part 2: Absorption Method
3. GB 4789.43-2016 National Food Safety Standard - Microbiological Examination of Food - Determination of Antibacterial Activity of Microbial Enzyme Preparations
Tryptone soy broth medium (TSB)Soy peptone broth is used for microbial enrichment cultivation. Tryptone and soy peptone provide carbon and nitrogen sources as well as other substances required for microbial growth; Sodium chloride maintains osmotic pressure.
Tryptone soy broth medium (TSB)Soy peptone broth is used for microbial enrichment cultivation. Tryptone and soy peptone provide carbon and nitrogen sources as well as other substances required for microbial growth; Sodium chloride maintains osmotic pressure.
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