There are nearly 3000 kinds of rat intestinal microvascular endothelial cells from Shanghai Beili Biological Company, which are from ATCC in the United States. The cells are all under strict quality control and strive to build a bridge between authentic cells and domestic researchers in Chinese Mainland. In addition, there are more related experimental products, standard products, cell lines, experimental technology services, and other pre - and follow-up services.
Rat intestinal microvascular endothelial cellsIntroduction:
source of organizationUsually isolated from normal small intestine tissue of rats within 24 hours of birth, such as the jejunum1.
Cell morphology and growth characteristicsLike endothelial cells, it is a flat polygon with serrated edges that interlock with each other. It grows on the surface of culture dishes and other containers by adhering to the wall25.
Material exchange barrierAs an important barrier for the exchange of substances inside and outside microvessels, it can selectively allow certain substances to pass through, such as nutrients and metabolic waste, while preventing harmful substances from entering tissues and maintaining a stable intestinal environment1.
Inflammation regulationPlays a gatekeeper role during inflammation, recruiting circulating immune cells and limiting inflammation. Under the action of cytokines and bacterial products, cell adhesion molecules can be activated and expressed, producing chemokines and increasing the attachment and movement of white blood cells3.
Immune response regulationHas strong immune reactivity to lipopolysaccharides (LPS) and plays a key regulatory role in the immune response of the digestive tract, participating in the immune defense process of the intestine3.
culture conditionsGenerally, M199 or DMEM medium is used, with the addition of 10% fetal bovine serum, insulin, transferrin, penicillin, streptomycin, etc., and cultured in an incubator with a gas phase of 95% air, 5% carbon dioxide, and a temperature of 37 ℃14.
Appraisal methodVWF immunofluorescence staining is commonly used. If cells show positive fluorescence, they can be identified as microvascular endothelial cells. In addition, markers such as Factor VIII and CD31 can also be used for identification13.
Rat intestinal microvascular endothelial cellsIt plays an important role in the study of intestinal physiology and pathological processes, such as investigating intestinal inflammatory diseases, intestinal tumor angiogenesis mechanisms, etc. It can also be used for drug screening and development, providing theoretical and experimental basis for the treatment of related diseases.
