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No. 15, Lane 88, Zhengyue Road, Yangpu District, Shanghai
Shanghai Weiting Trading Co., Ltd
No. 15, Lane 88, Zhengyue Road, Yangpu District, Shanghai
Attention: The products sold by our companySupplier of Human Thrombomodulin ELISA Kit Fbg reagent kitIt is only used for scientific research experiments and cannot be used for food or clinical use, edible drugs, etc.
While ensuring quality, the company,Supplier of Human Thrombomodulin ELISA Kit Fbg reagent kitWith the aim and concept of competitive prices, fast delivery, thoughtful service, and honest transactions, we are committed to various biological reagents, antibodiesELISASales promotion and service work for reagent kits, cytokines, and consumables. The company provides free testing services to better serve you. For details, please consult or consult online personnel.Imported original reagent kits require a delivery time of two to three weeks. If you need to place an order,You can consult or place an order through the following methods, and we sincerely hope to cooperate with you:
Specification: Original/96TELISAtest kitdomestic48T/96T Elisatest kit
Our company specializes in supplying various*ElisaThe quality of the reagent kit has been recognized by research institutions in major universities across the country and isElisaAs a long-term supplier of reagent kits and strategic partner. WelcomecomedianRequest for eachELISAProduct manual for reagent kit. All purchases of our company's catalogELISAThe testing kit can provide free proxy testing services. To place an order, please click on the button in the upper left cornerOnline Customer ServiceWe will consult with you as soon as possibleallyxi!
ELISAOperation steps of reagent kit
1 Balance from room temperature20minTake out the required Flat noodles from the rear aluminum foil bag, and seal the remaining Flat noodles with a self sealing bag4℃.
2 Set up standard wells and sample wells, and add different concentrations of standard samples to each standard well50ML;
3 Add the test sample to the hole first10MLAdd sample diluent40ML;
4 Subsequently, horseradish peroxidase was added to each well of the standard and sample wells(HRP)Labeled detection antibodies100MLSeal the reaction hole with a sealing film,37℃Incubate in a water bath or constant temperature box60min.
5 Discard the liquid, pat dry on absorbent paper, fill each hole with detergent, and let it stand1minDiscard the detergent, pat dry on absorbent paper, and repeat the washing process5The board can also be washed using a board washing machine.
6 Add substrate to each wellATheBeach50ML,37℃Incubate in the dark15min.
7 Add termination solution to each well50ML,15minInside, within450nmMeasure the wavelength of each holeODValue.
result judgment
Draw standard curve: inExcelIn the worksheet, use the standard concentration as the horizontal axis, corresponding toODDraw a linear regression curve of the standard sample with values as the vertical axis, and calculate the concentration values of each sample according to the curve equation.
Weiting Biotechnology Co., LtdI acted as an agentsigma,R&D,Hycult,Abnova,Abcam,BD,ScienCell,ATCC, Waiting for products from various foreign companies. Affordable prices, guaranteed quality, and top reputation.Imported original reagent kits require a delivery time of two to three weeks. If you need to place an order,PleasecomedianConsult or consult online personnel.While ensuring quality, the company,Supplier of Human Thrombomodulin ELISA Kit Fbg reagent kitWith the aim and concept of competitive prices, fast delivery, thoughtful service, and honest transactions, we are committed to various biological reagents, antibodiesELISASales promotion and service work for reagent kits, cytokines, and consumables. The company provides free testing services to better serve you. For details, pleasecomedianConsult or consult online personnel.