Human lymphocyte function associated antigen 3 ELISA kit

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Attention: The products sold by our companyHuman lymphocyte function associated antigen 3 ELISA kitImport LFA-3/CD58 assay kitOnly used for scientific research experiments, not for food or clinical use, edible drugs, etc

Shanghai Weiting Biotechnology Co., Ltd. acts as an agent for different brands of biological reagents,ELISAtest kit Standard products, reference materials, biological reagents, scientific research antibodies, cell lines, biological consumables, etc. Welcome toDian requests eachELISAProduct manual for reagent kit.Imported original reagent kits require a delivery time of two to three weeks. If you need to order:Human lymphocyte function associated antigen 3 ELISA kitImport LFA-3/CD58 assay kit,Please comeDian consultation or online customer service consultation.

ELISAPrecautions for operating the reagent kit

1. The reagent kit is stored in2-8℃Use front room temperature balance20minute The concentrated washing solution taken out of the refrigerator will have crystals, which is a normal phenomenon. Heating in a water bath will dissolve the crystals before use.

2. The Flat noodles not used in the experiment should be immediately put back into the self sealing bag, sealed (low-temperature drying) and stored.

3. The standard dilution solution can be considered as a negative control or blank; The preprocessed sample does not need to be diluted and can be taken directly10MLJust add samples.

4. Strictly follow the time, liquid dosage, and sequence indicated in the instructions for incubation operation.

5. Shake all liquid components thoroughly before use.

Preparation of reagents

20×Dilution of washing buffer: distilled water1：20Dilution, i.e1The share of20×Add washing buffer solution19Distilled water.

Washing method

1. Hand washed board: Shake off the liquid in the holes, fill each hole with detergent, and let it stand1minAfter shaking off the liquid in the hole, pat dry on absorbent paper and wash the board in this way5Next time.

2. Automatic washing machine: Inject washing solution into each hole350ML, soaking1minWash the board5Next time.

operating steps

1. Balance from room temperature20minTake out the required Flat noodles from the rear aluminum foil bag, and seal the remaining Flat noodles with a self sealing bag4℃.

2. Set up standard wells and sample wells, and add different concentrations of standard samples to each standard well50ML；

3. Add the test sample to the hole first10MLAdd sample diluent40ML；

4. Subsequently, horseradish peroxidase was added to each well of the standard and sample wells（HRP）Labeled detection antibodies100MLSeal the reaction hole with a sealing film,37℃Incubate in a water bath or constant temperature box60min.

5. Discard the liquid, pat dry on absorbent paper, fill each hole with detergent, and let it stand1minDiscard the detergent, pat dry on absorbent paper, and repeat the washing process5The board can also be washed using a board washing machine.

6. Add substrate to each wellATheBeach50ML，37℃Incubate in the dark15min.

7. Add termination solution to each well50ML，15minInside, within450nmMeasure the wavelength of each holeODValue.

result judgment

Draw standard curve: inExcelIn the worksheet, use the standard concentration as the horizontal axis, corresponding toODDraw a linear regression curve of the standard sample with values as the vertical axis, and calculate the concentration values of each sample according to the curve equation.

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