Since the discovery of green fluorescent protein in the 1960s, various fluorescent proteins (FP) and their variants have been developed, spanning the entire visible spectrum. The fluorescent proteins encoded by these genes have sparked a revolution in the field of cell biology. Blue green fluorescent protein (CFP) has received widespread attention due to its applications in multi-color fields such as FRET. This is because the wavelength range of CFP excitation and emission light is rarely used, making it an ideal choice for multiplexing various fluorescent groups.