In recent years, significant breakthroughs have been made in using in vitro tumor cell aggregates as a model to simulate the in vivo tissue environment. This technology enables discrete cells planted in low adsorption circular bottom well plates to aggregate into spheres. And spheres are believed to be able to simulate tumor behavior more effectively than conventional two-dimensional (2D) cell cultures.

In biological research and drug discovery, there is an increasing need to expand the diversity and complexity of cell analysis. Stem cell-derived cells and tissues are increasingly becoming an alternative to traditional in vitro and in vivo experiments in drug development and toxicological safety assessment. In this study, we used human iPSC derived myocardial and neuronal cell models to develop functional and morphological detection results, and tested the effects of different compounds in a multi parameter analysis format.

Macrophages originate from monocytes in the blood, which leave the bloodstream and differentiate into different tissues. Macrophages participate in the detection and phagocytosis of bacteria and damaged cells. In addition, macrophages trigger inflammation by releasing cytokines, which activate vascular cells, promote cytokine adhesion to blood vessels, and migrate to tissues. Differentiated THP-1 cells have been widely used in vitro macrophage models to study the involvement of macrophages in inflammatory responses.

Since the discovery of green fluorescent protein in the 1960s, various fluorescent proteins (FP) and their variants have been developed, spanning the entire visible spectrum. The fluorescent proteins encoded by these genes have sparked a revolution in the field of cell biology. Blue green fluorescent protein (CFP) has received widespread attention due to its applications in multi-color fields such as FRET. This is because the wavelength range of CFP excitation and emission light is rarely used, making it an ideal choice for multiplexing various fluorescent groups.

High connotation phenotype analysis is increasingly being applied in many research fields, including gene function research, drug development, and toxicology. The advantage of this method is that it objectively obtains multidimensional information from a single level, can analyze and describe the state of individual cells, and compare it with the overall analysis data. However, many traditional phenotype analyses subjectively select only a few important indicators to analyze in the pathway to be studied.

During clinical trials and after drug launch, cardiomyocyte toxicity remains the main cause of drug depletion. Although it is believed that a large proportion of cardiovascular diseases are caused by the environment; However, the causative environmental factors still have little to no distinctive characteristics. Therefore, how to detect potential myocardial toxicity is a crucial aspect of improving in vitro drug screening methods.

Image based phenotype analysis methods, such as the widely used Cell Painting analysis, use high connotation imaging and multi parameter outputs to study biological, genetic, and chemical disturbances in cells.

Organoid models are becoming increasingly popular in biological research and screening due to their ability to reproduce the complexity of real tissues. In order to simulate the human lung organs in vivo, we cultured primary human lung epithelial cells under conditions conducive to 3D structure formation, reproducing the morphological and functional characteristics of the lung airway. In lung organoid culture, epithelial stem cells and progenitor cells are cultured in ECM supplemented with a series of growth factors.

Angiogenesis is a physiological process in which new blood vessels and capillaries are formed and reshaped from pre-existing blood vessels. This can be achieved through budding or division of endothelial cells in blood vessels and capillaries. Vascular cells respond to appropriate stimuli by degrading the extracellular matrix, subsequently inducing endothelial cell proliferation and migration.

From studying diseases to evaluating toxic reactions induced by drugs and environmental compounds, cell-based assays typically focus on mitochondria and aim to monitor cellular health. Mitochondrial function is a key indicator of overall cellular health, and mitochondrial dysfunction is closely associated with various diseases, including Parkinson's disease, Alzheimer's disease, heart failure, ischemic diseases, and cancer.

In recent years, the demand for using more precise 3D cell models in physiology for research and drug discovery has been steadily increasing. Researchers have been building and maintaining various 3D cell models to study more diseases and physiological mechanisms [1,2].

Angiogenesis, the process of forming new blood vessels from existing blood vessels, is an important physiological phenomenon involving vertebrate development and wound healing. The process of angiogenesis is controlled by the action of pro angiogenic and anti angiogenic molecules under strict steady-state regulation. This disruption of internal homeostasis has been proven to play a role under various pathological conditions.