microbial breeding

1、 Product Introduction

Microbial breeding——Compared with the traditional low-pressure gas discharge plasma source, atmospheric room temperature plasma (ARTP) has the characteristics of low plasma jet temperature, uniform discharge and high concentration of chemically active particles. Based on ARTP technology, our company, together with relevant teams of Tsinghua University, has developed a special instrument for mutation breeding of microorganisms by means of plasma - ARTP Mutagenesis Breeding Machine. The instrument has a high mutation rate, compact structure, easy operation, high safety, and fast mutation speed. A large capacity mutation library can be obtained in one mutation operation (within a few minutes), greatly improving the intensity and capacity of strain mutation; ARTP technology combined with high-throughput screening technology can achieve rapid and efficient evolutionary breeding of organisms.

Microbial breeding——Non GMO methods ensure the safety of organisms

Wide range of applications, high mutation performance

Exclusive helium plasma mutagenesis technology, high energy, strong gene damage intensity

Easy to operate, safe, easy to maintain, and low operating costs

2、 Application areas:
Prokaryotes (such as bacteria, actinomycetes, etc.), eukaryotes (such as molds, yeasts, algae, higher fungi, etc.), and plant cells.
As of October 21, 2022, there are 411 Chinese literature and 169 English literature, patents269 papers, 176 dissertations, totaling 1025 papers
3、 Technical parameters

4、 Application case:
Case 1: Application of ARTP mutagenesis to induce high production of pyrroloquinoline quinone in Pseudomonas aeruginosa AM1

Figure 4 Batch Fermentation Results of M. extorquens AM1 Wild Strain (A) and Positive Mutant E-F3 (B) Fig. 4 Batch fermentation of M. extorquens AM1 (A) and E-F3 (B).

Using atmospheric pressure room temperature plasma (ARTP) for mutagenesis, combined with high-throughput rapid screening methods, a forward mutant strain with PQQ yield as the indicator was obtained. The strain induced by ARTP had a positive mutation rate of 31.6%, and the selected superior positive mutant strain M. extorquens AM1 (E-F3) achieved a yield of 54.0 mg/L, nearly three times that of the starting strain. (Journal of Bioengineering, 2016, 32 (8): 1145-1149)
Case 2: Application of ARTP to enhance the enzymatic activity of glutaminase produced by Streptococcus mutans

Typical colony characteristics after ARTP mutagenesis (G1-G14 represents typical colonies with different morphology from the starting strain); G15 has the same morphology as the starting strain)

The ARTP technique was used to induce mutations in Streptomyces spores, with a mutation rate of 42.8% and a positive mutation rate of 20.6%. The G2-1 enzyme activity of the high-yield mutant strain reached 2.73U/mL, which was higher than that of the starting strainimprove82%. (Microbiology Bulletin, 2010, 37 (11): 1642-1649)