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Wuxi Yuanqing Tianmu Biotechnology Co., Ltd
High throughput skin upgrade droplet single-cell sorter
NegotiableUpdate on 01/04
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Overview
Droplet Entrapping Microflfluidic Cell Sorter is an ultra high throughput single-cell sorting platform developed based on droplet microfluidic technology
Product Details
1、 Product Introduction
High throughput skin upgrade droplet single-cell sorting system（Droplet Entrapping Microfluidic Cell-sorter， DREM cell） It is a high-throughput single-cell sorting platform developed based on droplet microfluidic technology. It can generate thousands of micro droplets (pL) per second, in which cells are encapsulated and can undergo biological and biochemical processes such as growth, lysis, metabolism, and reaction. It also fully binds with the fluorescent sieve in the droplets to produce fluorescence signals of different intensities; Afterwards, using micro droplet sorting technology, low and high yielding cells were sorted out through fluorescence signals, achieving high-throughput quantification of the sorting process.
2、 Product advantages
3、 Application scenarios:
4、 Technical parameters:
| serial number | classification | Technical Specifications |
| 1 | Droplet diameter | 20-40μm |
| 2 | Droplet generation speed | Up to 10000/s |
| 3 | Droplet microinjection velocity | Up to 1000 pieces/s (optional) |
| 4 | Droplet sorting speed | Up to 1000/s |
| 5 | Temperature control in the sample area | 4 ℃± 0.5 ℃ constant temperature control |
| 6 | detection method | Fluorescence detection |
| 7 | Fluorescence excitation/emission wavelength | 405/460nm；488/525nm； 532/578nm; 561/620nm, etc., customizable |
| 8 | Scope of application | Bacteria, yeast, cells, proteins, nucleic acids, etc |
5、 Application Cases
Case 1: Directed Evolution of D-Aloulose-3-Isomerase Synthesis
A high-throughput screening platform based on droplet microfluidics (DREM cell) was constructed and combined with a D-aloulose biosensor for the directed evolution of ketose 3-isomerases (KEases); The catalytic efficiency of obtaining mutant M3-2 is 17 times higher than that of the original sfDAE
—Applied Chemistry, 2023
Case 2: High throughput screening of high-yield glutamine mutant strains
Upgrade using high-throughput skinsDroplet microfluidic cell sorter（DREM cell）， Ultra high-throughput screening was conducted on the whole genome mutation library of Bacillus amyloliquefaciens induced by atmospheric pressure room temperature plasma (ARTP), and a mutant strain with a glutamine enzyme production increase of over 47% was successfully obtained
—— Biochemical Engineering，2022
Case Three:Mining of genes associated with efficient secretion proteins of Bacillus subtilis
A genotype phenotype association map of protein secretion was drawn in the cereal stick, systematically revealing the gene loci in its genome that can be used to improve secretion protein production. A chassis strain with a 2.78-fold increase in VHH production was obtained by knocking out the combination of oxygen reducing transcription factors CosR and RshA
—— Metabolic Engineering，2022
Case 4: Study on the Functional Diversity of Bee Intestinal Microorganisms in Single Cell Culture
This study utilized a high-throughput droplet microfluidic platform (DREM cell) to perform skin upgraded droplet culture and niao gun based metagenomic sequencing on individual bacteria in the bee gut. Through metagenomic analysis, the diversity of bee gut microbiota at the strain level was demonstrated, revealing the existence of potential new strains in different bacterial genera
——Microbiome, 2022
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