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Wuxi Yuanqing Tianmu Biotechnology Co., Ltd
High throughput micro upgrade droplet culture omics system
NegotiableUpdate on 01/04
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Overview
The high-throughput upgraded droplet culture omics system (MISS cell omics system) is a miniaturized high-throughput single-cell culture and sorting equipment developed based on droplet microfluidics technology. The high-throughput upgraded droplet culture omics system can be used for the isolation and cultivation of environmental microorganisms, as well as the monoclonal culture and picking of single celled bacteria and filamentous bacteria (spores)
Product Details
1、 Product Introduction
High throughput micro upgrade droplet culture omics system(single cell microliter-droplet culture omics system， MISS cell omics system） It is a miniaturized high-throughput single-cell culture and sorting equipment developed based on liquid based microfluidic technology, which can separate and culture environmental microbial communities at the single-cell level, and sort and store them in porous plates to form a dual backup. A single run can process approximately 5000 droplets (500 monoclonal), which are then stored in a highly permeable pipeline for incubation (0-3 days). Finally, optical signals (OD, fluorescence, chemiluminescence, etc.) are used for detection and sorting. The sorted droplets enter a porous plate and can form a dual backup.
OD、 Online detection of fluorescence, chemiluminescence, etc
In situ cultivation of high gas mass transfer
Filter to standard porous plate
Single clone dual backup
Support single clone picking of filamentous bacteria
2、 Product application
Isolation and cultivation of environmental microorganisms, monoclonal cultivation and selection of single celled bacteria and filamentous bacteria (spores).
3、 Product parameters
| classification | Technical Specifications |
| microfluidic chip | Biocompatible polymer chip material |
| Micro droplet volume | 1-4 μL |
| Droplet cultivation container | Multiple gas permeable polymer materials; Controllable oxygen partial pressure environment (optional) |
| Droplet generation flux | 5000-10000 pieces/h |
| Droplet detection flux | 1800-2500 units/h |
| Droplet sorting | 1800-2500 units/h |
| optical inspection | OD、 Fluorescence, chemiluminescence, etc., multi band simultaneous excitation and detection |
| Droplet cultivation time | 0-8 days |
| Scope of application | Single celled microorganisms (bacteria, yeast, microalgae), filamentous bacteria (molds, actinomycetes), etc |
4、 Workflow of high-throughput micro upgrade droplet culture omics system:
Sample preparation, droplet generation, droplet cultivation
Droplet collection to porous plate, droplet backup, droplet detection and sorting
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