VERO African Green Monkey Kidney Cells

VERO-K African Green Monkey Kidney Cells， ATCC cells | cell lines | cell lines | tumor cells | cells | adherent cells | suspended cells |, standardized cell bank management, clear background of provided cell lines, references and optimal culture conditions

VERO African Green Monkey Kidney CellsDetailed Introduction

Cultivation conditions:

Culture medium: DMEM high glucose medium 90%; 10% fetal bovine serum.

Cultivation conditions: 37.0C carbon dioxide (CO2), 5%

Passage method:

After receiving the cells, take out the culture bottle and observe the cell growth under an inverted microscope.

（1） If the cells are not fully grown, spray the entire bottle with 75% alcohol for disinfection and place it in a super sterilization table. Perform strict aseptic operation, open the cell culture bottle, aspirate the culture medium, and leave only 10ml of culture medium in the bottle for further cultivation.

（2） If the cells are already fully grown, they can be passaged and cultured. The specific steps are as follows:

1. Discard the culture medium and wash 1-2 times with PBS (free of calcium and magnesium ions).

2. Add 1ml of digestion solution (0.25% Trypsin 0.53mM EDTA) to a culture bottle, invert and place in a 37 degree incubator for 1-3 minutes to preheat. Then invert the culture bottle for about 30 seconds and observe the cell digestion under an inverted microscope. If most of the cells become round and dispersed, tap the culture bottle lightly and the cells will immediately fall off.

3. Add 6-8ml of culture medium, aspirate, and transfer to new culture bottles. 1: 3~1:6 passage; Once every 2-3 days.

Attention: After passaging, use half of our culture medium and the other half of yours to avoid the cells not adapting and causing damage

Growth is not good.

Freezing method: Freezing solution: Basic culture medium+5% DMSO+20 ° S

Storage: Liquid nitrogen storage