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IMR-90 human embryonic lung fibroblasts
NegotiableUpdate on 05/06
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Overview
IMR-90 human embryonic lung fibroblast cells, primary human embryonic lung fibroblast cells | cell lines | cell strains | strains, standardized cell bank management, clear cell line background provided, references and * culture conditions provided!
Product Details
IMR-90 cells (IMR-90 human embryonic lung fibroblasts) ATCC ® CCL-186 ™ Detailed information
Basic Information
- ATCC number：CCL-186™
- designation：IMR-90
- depositorsWW Nichols (W.W. Nichols)
- Biosafety levelLevel 1 (BSL-1)
- Mode of transportationFreezing (dry ice)
- Growth characteristics: adherent growth
- genus: People（Homo sapiens）
- form: Fibroblast like
- source of organization: Lung
- disease state: Normal
- cell type: Fibroblasts
Licensing and Regulations
In addition to the Material Transfer Agreement (MTA) mentioned above, transferring the ATCC material may require other ATCC and/or regulatory permits. The user who purchases ATCC materials is ultimately responsible for obtaining such licenses. For specific requirements for shipping to your region, please click here to inquire.
application
- Transfect the host (applicable to Lonza's Nucleofection technology).
Virus susceptibility
This cell is sensitive to the following viruses:
- Human poliovirus 1
- Human poliovirus 2
- Varicella Zoster virus
- Herpes simplex virus 1
- Herpes simplex virus 2
- Human poliovirus 3
- Vaccinia virus
- Human herpesvirus 5 (CMV)
- Vesicular stomatitis virus
molecular characteristics
- Reverse enzyme activity: Negative (Reverse Script: Negative)
- STR DNA typing(Short serial repeat sequence):
- Amelogenin (gender locus): X
- CSF1PO：11,13
- D13S317：11,13
- D16S539：10,13
- D5S818：12,13
- D7S820：9,12
- THO1：8,9.3
- Tpox ： 8, 9
- vWA：16,19
-
- Cytogenetic analysisNormal female diploid (46, XX), chromosome stable (normal human female; diploid; stable)
- isozymeGlucose-6-phosphate dehydrogenase (G6PD) type B (Isoenzymes: G6PD, B)
Source and Background
The IMR-90 cell line was developed by W. W. Nichols and colleagues from16 week old female fetusIt was isolated from normal lung tissue and is a human diploid fibroblast.
The division potential, viral susceptibility, and other characteristics of this cell have been extensively studied and can serve as an alternative to WI-38 (another commonly used human lung fibroblast cell line) and other standard human lung cell lines.
According to reports, IMR-90 cells exhibitThe population doubling frequency (PDL) is approximately 58 times.
culture conditions
1. Complete growth medium
The basic culture medium is Eagle's Minimum Essential Medium prepared by ATCC, item number 30-2003.
When preparing the complete culture medium, it is necessary to add it to the basic culture medium10% fetal bovine serum (FBS).
2. Cultivation environment
- Gas atmosphereAir (95%)+Carbon Dioxide (CO ₂, 5%)
- temperature：37.0°C
3. Subculture
operating steps：
- Remove and discard the old culture medium.
- Wash the cell layer briefly with 0.25% (w/v) trypsin 0.53 mM EDTA solution to remove serum residues containing trypsin inhibitors.
- Add 2.0-3.0 ml trypsin EDTA solution to the culture bottle and observe under an inverted microscope until the cell layer is dispersed (usually taking 5-15 minutes).Caution: Avoid shaking or tapping the culture bottle to prevent cell clumping. Cells that are difficult to disperse can be placed in a 37 ° C incubator to accelerate dissociation.
- Add 6.0-8.0 ml of complete growth medium and gently blow the cells to terminate trypsin digestion.
- Transfer the cell suspension into a new culture container (such as a culture bottle or dish).
- Cultivate in a 37 ° C, 5% CO ₂ incubator.
Passage ratioRecommend a ratio of 1:2 to 1:8 (i.e. dividing 1 cell suspension into 2-8 new containers).
Frequency of medium replacementChange the fresh complete culture medium every 3-4 days.
4. Preservation
- cryopreservation solutionComplete growth medium (95%)+dimethyl sulfoxide (DMSO, 5%).
- Storage temperatureLiquid nitrogen vapor phase.
Related Products
- Recommended Basic Culture Medium(Excluding additional supplements or serum): ATCC 30-2003 (Eagle's Minimum Essential Medium).
- Recommended serumATCC 30-2020 (fetal bovine serum).
References
- Nichols WW, et al. Characterization of a new human diploid cell strain, IMR-90.Science196: 60-63, 1977.（PubMed: 841339）
- Dolganov GM, et al. Human Rad50 is physically associated with human Mre11: identification of a conserved multiprotein complex implicated in recombinational DNA repair.Mol Cell Biol16: 4832-4841, 1996.（PubMed: 8756642）
- Ostlund RE Jr., et al. A stereospecific myo-inositol/D-chiro-inositol transporter in HepG2 liver cells.J Biol Chem271: 10073-10078, 1996.（PubMed: 8626564）
Other information
- Cell bank information: The cell bank (GNHu32) of the Committee for the Preservation of Typical Cultures of the Chinese Academy of Sciences provided this cell, which was negative for mycoplasma, bacteria and fungi, and passed STR identification.
- Supply restrictionsFor research use only.
IMR-90 cells, human embryonic lung fibroblasts
ATCC® Number: CCL-186™
Designations: IMR-90
Depositors: WW Nichols
Biosafety Level: 1
Shipped: frozen
Medium & Serum: See Propagation
Growth Properties: adherent
Organism: Homo sapiens (human)
Morphology: fibroblast
Source: Organ: lung
Disease: normal
Cell Type: fibroblast
Permits/Forms: In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimay responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
Applications: transfection host (Nucleofection technology from Lonza)
Virus Susceptibility: Human poliovirus 1
Human poliovirus 2
Varicella-Zoster
Herpes simplex virus 1
Herpes simplex virus 2
Human poliovirus 3
Vaccinia virus
Human herpesvirus 5
Vesicular stomatitis virus
Reverse Transcript: negative
DNA Profile (STR): Amelogenin: X
CSF1PO: 11,13
D13S317: 11,13
D16S539: 10,13
D5S818: 12,13
D7S820: 9,12
THO1: 8,9.3
Tpox: 8, 9
vWA: 16,19
Cytogenetic Analysis: normal human female; diploid; stable
Isoenzymes: G6PD, B
Age: 16 weeks gestation
Gender: female
Ethnicity: Caucasian
Comments: The human diploid fibroblast strain IMR-90 was derived by W.W. Nichols and associates from the lungs of a 16-week female fetus. [22381]
The division potential, viral susceptibilities and other properties have been thoroughly studied such that the line may be considered as an alternate for WI-38 and other standard human lung cell strains.
The cells have been reported to be capable of attaining 58 population doublings before the onset of senescence. [22381]
Propagation: ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Eagle's Minimum Essential Medium, Catalog No. 30-2003. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Atmosphere: air, 95%; carbon dioxide (CO2), 5%
Temperature: 37.0°C
Subculturing: Protocol:
Remove and discard culture medium.
Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.
Add 2.0 to 3.0 ml of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).
Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.
Add 6.0 to 8.0 ml of complete growth medium and aspirate cells by gently pipetting.
Add appropriate aliquots of the cell suspension to new culture vessels.
Incubate cultures at 37°C.
Subc*tion Ratio: A subc*tion ratio of 1:2 to 1:8 is recommended
Medium Renewal: Every 3 to 4 days
Preservation: Freeze medium: Complete growth medium 95%; DMSO, 5%
Storage temperature: liquid nitrogen vapor phase
Related Products: Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2003
recommended serum:ATCC 30-2020
References: 22381: Nichols WW, et al. Characterization of a new human diploid cell strain, IMR-90. Science 196: 60-63, 1977. PubMed: 841339
32932: Dolganov GM, et al. Human Rad50 is physically associated with human Mre11: identification of a conserved multiprotein complex implicated in recombinational DNA repair. Mol. Cell. Biol. 16: 4832-4841, 1996. PubMed: 8756642
33041: Ostlund RE Jr., et al. A sterospecific myo-inositol/D-chiro-inositol transporter in HepG2 liver cells. J. Biol. Chem. 271: 10073-10078, 1996. PubMed: 8626564
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