TDCCS-3D Microgravity 3D Cell Culture System

Parabolic flight and tower descent are methods of creating microgravity environments on Earth, similar to the International Space Station (ISS) and China Space Station. However, whether it is parabolic flight or tower landing, they can only create a microgravity environment for a few seconds. In order to create a microgravity environment on Earth for a long time, scientists invented the "inclinometer" in the late 19th century, initially to study the response of plants to gravity. The "inclinometer" is a horizontally rotating machine that fixes the sample on a machine that rotates around a horizontal axis to suppress unidirectional gravity loads. This is the original 1-axis (1D) inclinometer, which allowed researchers to study the effects of gravity in ground-based laboratories for a long time, but it could not create a microgravity environment. Therefore, the 3D inclinometer was born. The 3D inclinometer has two rotation axes, which can achieve three-dimensional rotation; Allow unidirectional gravity to disperse in different directions to create a simulated microgravity environment. TDCCS-3D has emerged.

TDCCS-3D Microgravity 3D Cell Culture SystemIt is a novel low shear culture system designed for adherent cells and suspended cells. Based on existing domestic and foreign literature, it is clearly demonstrated that gravity has a significant impact on cell proliferation and differentiation. Cultivating cells in a micro heavy environment has shown significant improvements in both cell quality and passage quantity; However, due to the scarcity of space station resources and high cost investment, many scientific research ideas are unable to proceedCan be implemented; Therefore, we believe that the TDCCS-3D series universal multi axis rotary system developed by Beijing Keyu Xingye Company, as a new experimental equipment, can provide researchers with a feasible alternative solution.

TDCCS-3D Microgravity 3D Cell Culture SystemBreaking through traditional structural design, a tilted 45 ° rotating device is used to achieve three-dimensional rotation of the entire machine, and low shear force is also achieved in the cell culture process. The TDCCS-3D microgravity 3D cell culture system also adds the function of "overweight", which is an intelligent 3D cell culture system that integrates both microgravity and supergravity modes. The overweight mode cell culture function of TDCCS-3D can promote cell differentiation; Can meet the experimental needs of researchers exploring the physicochemical properties of cells in overweight environments.

1. Accurate simulation of physiological environment: Using a tilted 45 ° rotating device to achieve three-dimensional rotation of the whole machine, it can effectively achieve low shear force during cell culture process, allowing cells to be in a free suspension state, accurately simulating microgravity environment, and closer to the mechanical microenvironment in which cells are located in the body. It also adds overweight function, which is an intelligent system that integrates microgravity and supergravity modes. It can simulate multiple gravity levels and monitor three-dimensional gravity, displaying gravity values in real time with an accuracy of ± 0.001g.

2. Better cultivation effect: Cells in this system can maintain gene expression profiles and drug response characteristics that are closer to those in vivo, forming structures similar to in vivo tissues, maintaining their original morphology, polarity, and function. The interactions between cells and between cells and extracellular matrix are also closer to the real situation in vivo. For example, neural stem cells can better form neurospheres, liver cells can form tight cell connections, and pancreatic beta cells can secrete insulin more stably.

3. Assist in deep cell research: It can be used for in-depth study of processes such as cell proliferation, differentiation, migration, apoptosis, as well as the mechanism of drug action on cells. In tumor research, simulating the growth and metastasis process of tumor cells in vivo can help researchers clearly observe the invasion and metastasis mechanisms of tumor cells.

4. Improve drug development efficiency: In drug screening and toxicology research, cultured cells are closer to the in vivo state, making research results more accurate and reliable, and better predicting the efficacy and toxicity of drugs in vivo, greatly reducing the cost and risk of drug development. According to experimental data, using this system for drug screening can improve the accuracy of drug efficacy evaluation by 30%.

Easy to operate and widely applicable: suitable for various cell types, whether they are adherent cells, suspended cells or primary cells, they can grow well in this system, widely meeting different research directions and experimental needs.