Streck218962 gene non-invasive tube

Streck gene non-invasive tube DNA collection tube is a direct whole blood collection tube used for collecting, stabilizing, and transporting free plasma DNA. The device also stabilizes and preserves cellular genomic DNA present in nucleated blood cells and circulating epithelial cells (tumor cells) found in whole blood. This preservative can prevent the release of genomic DNA, allowing for the isolation of high-quality cell-free DNA

Streck218962 gene non-invasive tubeProtecting cell surface antigens, maintaining cell morphology, and preventing the release of nucleic acids from blood cell rupture can ensure the extraction of plasma free DNA and facilitate downstream analysis experiments. Samples collected in plasma free DNA collection and preservation tubes can be stored at 6-37 ℃ for up to 14 days, and transported at room temperature for up to 72 hours, greatly facilitating the collection, transportation, and preservation of blood samples. The plasma free DNA collection and preservation tube is suitable for various clinical studies, drug development, and molecular diagnostics.

Streck218962 gene non-invasive tubePrinciple:

Nucleic acid testing in blood, including detection and analysis of free DNA in plasma, is an emerging and reliable field in disease diagnosis. Although there is only a small amount of free DNA in healthy human plasma, the increase or decrease of free DNA in the blood circulation is related to many clinical diseases. Research has shown that the Stress non-invasive tube, as a non-invasive, rapid, and sensitive diagnostic tool, can be applied for molecular diagnosis and detection of acute diseases, as well as prenatal diagnosis of fetal genetic diseases. During the analysis of free DNA, the handling, transportation, and processing of the sample are highly susceptible to contamination, leading to the breakdown of nucleated blood cells and further release of cellular genomic DNA. Meanwhile, the degradation of free DNA caused by nuclease activity can also bring a series of problems.

Product ordering information

brand Item Number Product description packaging

Stretch 218962 Cell Free DNA Plasma Free DNA Sample Collection Vessel 100 tubes/box

Stretch 230253 Cell Free DNA Plasma Free DNA Sample Collection Vessel(Mixed plastic) 100 tubes/box

218962 gene non-invasive blood collection tube, DNA blood collection tube, Down syndrome prenatal screening NIPI、CTC

Product Application

1. NIPT non-invasive prenatal testing: Preservation of fetal free DNA from maternal peripheral blood and analysis of common fetal genetic diseases: Trisomy syndrome

2. Tumor liquid biopsy: preservation of circulating tumor cells (CTCs) and ctDNA (circulating tumor DNA)

3. Other: Preservation of plasma genomic DNA and free DNA

Storage and stability

Product Features

1. Time and effort saving: The STRECK non-invasive tube provides a solution for detecting and analyzing cell-free DNA in patient blood samples, which is more labor-saving and time-saving than traditional plasma sample preparation methods.

2. Convenient coordination: Samples in the STRECK non-invasive tube can be stored at room temperature for up to 14 days, making sample collection, transportation, and storage more convenient.

3. Widely applicable: The high-quality cell-free DNA obtained from STRECK non-invasive tubes can be used for various clinical medical research, drug discovery, and diagnostic testing.

4. Accurate results: The STRECK non-invasive tube requires minimal sample processing, enhances sample stability, and extracts cell-free plasma DNA, minimizing the risk of variation in cell-free DNA sample preparation.

218962 gene non-invasive blood collection tube, DNA blood collection tube, Down syndrome prenatal screening NIPI、CTC

noun explanation

1. NIPINon invasive prenatal screening is a method of using large-scale parallel sequencing and other technologies to deeply sequence free DNA in maternal peripheral blood and obtain fetal chromosome information. Traditional prenatal screening and diagnostic methods (early pregnancy ultrasound examination, mid pregnancy serological screening, amniotic fluid cell chromosome karyotyping analysis) have certain limitations, especially the puncture method may harm the fetus and lead to miscarriage. With the deepening of research on next-generation sequencing technology, non-invasive fetal genetic testing has developed rapidly.

2. CTC, also known as circulating tumor cells, typically refers to cancer cells that detach from clusters of cancer cells and enter the circulatory system;　　

3. CtDNA is called circulating tumor DNA. During the process of division and proliferation, tumor cells undergo apoptosis, death, and actively secrete DNA fragments that have undergone genetic mutations into the bloodstream. We call this DNA fragment ctDNA.

[Instructions for Use]

Direct extraction method

1. According to the description in CLSI H3-A61, samples were taken by venipuncture.

2. Draw blood in the order listed in CLSI H3-A61.

3. Fill the test tube

4. Remove the connecting tube from the test tube and immediately gently invert it 8 to 10 times to mix the liquid inside the tube (as shown in the figure). Insufficient mixing or delayed mixing can both lead to incorrect test results.

operating procedure

1. Collect blood into a test tube according to H3-A61 in the CLSI document.

2. After the collection is completed, please transport and store within the temperature range.

3. Extract cell-free DNA BCT according to the instructions of the instrument manufacturer.

Note:

1. The STRECK non-invasive tube does not dilute blood, therefore, there is no need to perform dilution factor correction to obtain absolute values.

2. As with most clinical trial specimens, hemolysis, jaundice, and lipemia can all affect the test results obtained by storing blood samples in the STRECK non-invasive tube.

Extraction of cell-free plasma DNA and cellular genomic DNA:

1. The extraction of cell-free plasma DNA and cellular genomic DNA can be accomplished using most commercially available assay kits.

2. To obtain results, a proteinase K treatment step (≥ 30 mAU/ml) is added to the 60 ℃ free solution salt. It takes 1 hour to extract cell-free DNA and 2 hours to extract cellular genomic DNA.

restriction

1. Unused test tubes should be stored between 18-30 ℃.

2. Samples extracted into other anticoagulants or preservatives may cause coagulation in the STRECK non-invasive tube.

reference resources:

Certification of Clinical Trial Standards Committee Standard Sixth Edition. H3-A6， Procedure for collecting diagnostic blood samples through venipuncture;

218962 gene non-invasive blood collection tube, DNA blood collection tube, Down syndrome prenatal screening NIPI、CTC

【 Precautions 】

1. Can only be used for scientific research, not for medical diagnosis.

2. The STRECK non-invasive tube containing the sample cannot be refrigerated to prevent the tube from rupturing.

3. Please use within the validity period of the test tube.

4. Test tubes cannot be used for collecting injectable materials.

5. Do not dilute or add other ingredients.

6. Excessive or insufficient filling of test tubes can cause an imbalance in the ratio of blood and additives, leading to incorrect analysis results or reduced product performance.

7. Warning

1. Glass may break, please take preventive measures during the handling process.

2. All biological specimens and materials in contact with the product will be considered as infectious biological hazards, and should be handled in accordance with relevant local regulations. Avoid contact with skin and mucous membranes.

3. This product should be disposed of as infectious medical waste.

4. When removing or reinstating the test tube stopper, gently shake the stopper or grip it while twisting and pulling outward. Do not use your thumb to push out the stopper, as it may cause the test tube to rupture and cause injury.

8. Prevent backflow

Due to the presence of chemical additives in the STRECK non-invasive tube, efforts should be made to prevent liquid from flowing out of the tube.

To prevent backflow, please follow the following preventive measures:

1. Please keep the patient's arm in a downward position during the collection process.

2. Try to hold the stopper upwards when holding the test tube.

3. Blood begins to flow into the test tube or the tourniquet is released two minutes after use.

4. During the collection process, objects inside the test tube should not come into contact with the stopper or needle.

STRECK Non Invasive Tube Storage and Transportation

1. At temperatures between 18-30 ℃, the STRECK non-invasive tube can be stored stably within its shelf life.

2. Do not refrigerate empty STRECK non-invasive tubes. Appropriate isolation measures should be taken during transportation under temperature conditions.

3. Blood samples collected in the STRECK non-invasive tube for cell-free DNA analysis can be stored stably for 14 days in an environment of 6-37 ℃.