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Shenzhen Xinbosheng Biotechnology Co., Ltd

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    Room 0605, Science and Technology Building, Duoli Industrial Zone, 105 Meihua Road, Shangmeilin, Futian District, Shenzhen

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SurModics substrate reagents

NegotiableUpdate on 05/06
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Overview

Enzyme substrate interactions are widely used in the detection of results in various immunoassays, such as Elisa, ELISpot, immunoblotting, and IHC. SurModics can provide chromogenic substrates and chemiluminescent substrates, which can be packaged in large quantities or according to customer requirements.

Product Details

Enzyme substrate interactions are widely used in the detection of results in various immunoassays, such as Elisa, ELISpot, immunoblotting, and IHC. SurModics can provide chromogenic substrates and chemiluminescent substrates, which can be packaged in large quantities or according to customer requirements.


Main products

number

name

specification

Storage conditions

Validity period

TMBW-0100-01

TMB single component HRP microplate chromogenic substrate

100ml

2°C - 8°C

48 months

TMBW-1000-01

TMB single component HRP microplate chromogenic substrate

1000ml

2°C - 8°C

48 months

PNPS-0100-01

Yellow single component pNPP AP microplate chromogenic substrate

100ml

2°C - 8°C

36 months

PNPS-1000-01

Yellow single component pNPP AP microplate chromogenic substrate

1000ml

2°C - 8°C

36 months

BCIB-0100-01

Application substrate of BCIP/NBT blue single component AP membrane

100ml

2°C - 25°C

36 months

BCIB-1000-01

Application substrate of BCIP/NBT blue single component AP membrane

1000ml

2°C - 25°C

36 months


SurModics底物试剂

Figure 1. PNPS

SurModics底物试剂


Figure 2. BCIB

The BioFX TMB HRP microplate substrate provides stable kinetic parameters for the application of horseradish peroxidase micropores. The characteristics of this type of substrate include: different substrates can be selected according to the detection level, and each substrate can provide stable and uniform results; Non toxic and harmless, without inert reagents; Zhuoyue's sensitivity can be maintained for a very long time; From colorless to blue.


BioFX PNPS is the only alkaline phosphatase pNPP chromogenic substrate suitable for microplates (patent number: 7001717). Containing phosphate p-nitrophenyl phenol (pNPP), a single component, ready to use form, the unreacted substrate appears colorless to light yellow, and after reacting with phosphatase, it turns yellow. Not recommended for products that require precipitation reactions such as membranes or IHC.


Comparing the BioFX AP Yellow single component microplate pNPP substrate with regular pNPP single component liquid, the results showed that the BioFX substrate had a high signal-to-noise ratio every day after storage at 37 ℃ for 0, 2, 4, 8, 15, and 30 days (see Figure 1).


BioFX BCIP/NBT substrates are available in two formulations, blue and purple, for membrane based detection of alkaline phosphatase. Single component, ready to use type. Insoluble, reacts with alkaline phosphatase to form blue color (see Figure 2).


For more details, please contact SurModics China agent - Xinbosheng Biotechnology