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Shanghai Yougaojiu Trading Co., Ltd
Streptavidin (ST II) protein resin 4FF
NegotiableUpdate on 05/06
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Overview
Strep Tag II is a small tag consisting of 8 amino acids (WSHPQFEK). Due to its small size, only around 1.06 kDa, it does not affect the structure and function of the fused protein. These mild purification parameters can maintain the biological activity of the protein and produce a purity of over 99% in just one step of extraction. Among numerous affinity tags, although Strep tag II appeared later than Flag, c-Myc, and His tags, it has high affinity, high purity in single step purification (better than His tag), mild purification conditions, and can be used at both ends of the protein
Product Details
Streptavidin (ST II) protein resin 4FF It can be used for purification of Strep Tag II tagged proteins in various expression systems, including rod-shaped viruses, mammalian cells, yeast, and bacteria. It is suitable for most protein properties and facilitates analysis of protein-protein interactions. At the same time, the resin can also be used for Twin Strep Tag II tag purification.
Model specifications
600698-1 1ML
600698-5 5ML
characteristic
The purification conditions are mild, and the purity of single step purification is better than that of His tags. It does not affect the structure and function of the fused protein, has high affinity, and can tolerate different buffer conditions and additives (such as high salt, detergent, reducing agent, metal ion, and chelating agent).
application
It can be used for purification of Strep Tag II tagged proteins in various expression systems, including rod-shaped viruses, mammalian cells, yeast, and bacteria. It is suitable for most protein properties and facilitates analysis of protein-protein interactions. At the same time, the resin can also be used for Twin Strep Tag II tag purification.
component
1 mL/5 mL
Applicable samples
Purification of Strep tag II and Twin Strep tag II proteins
attribute
4% agarose gel with highly crosslinked matrix
Ligand Streptactin Mutant
Loading capacity ≈ 4 mg Twin Strep tag II protein/mL filler
Particle size 45 μ m~165 μ m
Maximum flow rate 300 cm/h
Storage Buffer: 1X PBS solution containing 20% ethanol
Storage temperature 2-8 ° C, non freezing
Tolerance (within 2 hours) 6M guanidine hydrochloride, 8M urea, 2M NaCl
