- Phone
-
Address
Junlin Business B309, No. 233, Lane 655, Bao'an Road, Baoshan District, Shanghai
Product Categories
Shanghai Yougaojiu Trading Co., Ltd
GST 4FF (GST Tag) agarose purification resin
NegotiableUpdate on 05/06
- Model
- Nature of the Manufacturer
- Producers
- Product Category
- Place of Origin
Overview
GST 4FF (GST Tag) agarose purification resin derived from Schistosoma japonicum (GST) is a commonly used fusion tag in the design and expression of recombinant proteins in genetic engineering. After fusion with GST, the recombinant protein not only improves stability and solubility, but also brings great convenience to downstream separation and purification. The molecular weight of GST is approximately 26 kDa, and it has excellent affinity for reduced glutathione (GSH). Therefore, immobilized GSH can be used for one-step affinity purification of GST fusion proteins.
Product Details
GST 4FF (GST Tag) agarose purification resin
Model specifications
600031-5 5 ML
600031-10 10 ML
600031-25 25 ML
600031-100 100ML
characteristic
1. Good specificity and high affinity for GST fusion protein. The target protein with a purity of over 95% can be obtained by one-step affinity purification from bacterial lysate; 2. The GST fusion protein bound to the medium can be competitively eluted by high concentrations of reduced glutathione (GSH) (33 mM) in solution. For different GST fusion proteins, there is no need to explore optimal GSH elution concentrations, making the use of the medium simpler; 3. Renewable and reusable.
application
Used for separating and purifying soluble recombinant proteins with GST tags, inclusion body proteins dissolved in high concentration denaturing agents cannot be purified using this medium.
component
5 ml/10 ml/25 ml/100 ml
attribute
stroma:Highly cross-linked 4% agarose
Average particle size:90 μm（45-165 μm）
Protein binding ability:Approximately 5-10 mg GST tagged protein per 1ml
Maximum flow rate:300 cm/h
Ligand density: approximately 10-20 μ mol/ml GST resin
Pressure resistance: 0.3 MPa, 3 bar
Storage solution: 20% ethanol
Acid alkali stability: Short term (2 hours) pH 3-12
Storage temperature: 4-8 ° C, non freezing
