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Ni NTA 6FF agarose purification resin
NegotiableUpdate on 05/06
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Overview
The matrix of this medium is 6% highly cross-linked agarose with an average particle size of 90 μ m, and the metal ion chelating ligand has good stability through covalent connection. Chelating ligands with divalent Ni2+are the medium for purifying most His Tag tagged proteins. The ligand density of the medium has been optimized to ensure high protein binding loading and selectivity, while the high flow rate characteristics make the medium suitable for large-scale purification.
Product Details
Ni NTA 6FF (His Tag) protein agarose purification resin
Model specifications
600033-10 10 ML
600033-25 25 ML
600033-100 100ML
600033-200 200ML
600033-1000 1000ML
characteristic
Compared to Ni IDA agarose made from trivalent metal ion chelating agent iminodiacetic acid, Ni NTA agarose has significantly reduced non-specific adsorption, allowing users to obtain higher purity target proteins; 2. Support column refolding of denatured inclusion body proteins, achieving simultaneous separation and refolding effects; 3. Can tolerate a certain concentration of reducing reagents; 4. The agarose matrix remains stable within the pH range of 2-14 and can tolerate repeated in place cleaning (CIP); five Very little Ni2+leakage occurs during the purification process; six Can be reused and regenerated 5-10 times.
application
Used for various expression sources such as Escherichia coli, yeast, insect cells, and mammalian cellsHis-TagPurification of tag proteins.
component
10 ml/25 ml/100 ml/250 ml/1 L
attribute
Matrix 6% high cross-linked agarose
平均粒径 90 μm
Protein binding capacity~40 mgHis-TagLabel protein/1ml resin
Maximum flow rate 600 cm/h
Ligand density~25 μ mol/ml
Pressure resistance 0.3 MPa, 3 bar
Storage solution: 20% ethanol
Acid alkali stability 3-12 (working); 2-14 (CIP, nickel removal)
Storage temperature 4-8 ° C, non freezing
Common aqueous buffer solutions for chemical stability include 1 M NaOH, 70% acetic acid, 2% SDS, 30% isopropanol, 6M guanidine hydrochloride, and 8M urea
