Specialized basic culture medium for organoids

NegotiableUpdate on 05/06

Model

Nature of the Manufacturer: Producers

Product Category

Place of Origin

Online Consult

Overview

OC Advanced DMEM/F12 is an optimized basic culture medium used for culturing mammalian epithelial organoids. Serum substitute O27 (MB-23061A010) needs to be supplemented in the basic organoid culture medium. In addition, different cytokines, amino acids, small molecule inhibitors, etc. should be added as needed. This culture medium does not contain proteins or any other animal derived components.

Product Details

OC-Advanced DMEM/F-12 /OC-Advanced DMEM/F-12（Phenol Red Free）

Specialized basic culture medium for organoids

♢Low temperature needs to be maintained throughout the transportation of the product.

♢Suggest storing in2-8℃In the refrigerator.

♢Pay attention to aseptic operation when using.

1 Product Description

Specialized basic culture medium for model based biological organs（OC-Advanced DMEM/F12）It is an optimized basic culture medium used for mammalian epithelial organ culture. When applied to organoid culture, serum substitutes need to be supplementedO27(MB-230614A010)In addition, different cytokines, amino acids, small molecule inhibitors, etc. should be added as needed.OC-Advanced DMEM/F12It can also be widely applied in cell culture, usingOC-Advanced DMEM/F12When cultivating cells, fetal bovine serum（FBS）The amount of addition can be reduced50%-90%And there is no change in cell growth rate and morphology. Cells cultured in this medium usually do not require an adaptation period, such asMRC-5TheSP2TheVeroTheWI-38andJacketCells, etc.

2 Product Information

Product Name

Product Item Number

specification

store/transportation

shelf life

Specialized basic culture medium for organoids

MB-0930L500

500mL

2-8 °C

Avoid direct sunlight

12month

Specialized basic culture medium for organoids (excluding phenol red)

MB-0930L500F

3 Instructions for Use

a) Preparation of organoid culture medium

1 4°CTake out the organ specific basic culture medium from the refrigerator（OC-Advanced DMEM/F12）Restore it to room temperature.

2 Add to organoid specific basic culture medium2%ofO27Culture medium additives(MB-230614A010，50X)Make its final concentration1x,(For example: every500mLBasic culture medium needs to be added10mL O27）.

3 According to different organoid culture requirements, different cytokines, small molecule inhibitors, amino acids, hormones, and antibiotics are added in sequence.

4 After the preparation of organoid culture medium is completed, it can be used for4°CStable storage in the refrigerator for up to2Zhou, it is recommended to pack and place it in-20°CFreeze storage.

b) Preparation of cell culture medium

1 4°CTake out the organ specific basic culture medium from the refrigerator（OC-Advanced DMEM/F12）Restore it to room temperature.

2 According to different cell culture requirements, add different serum, antibiotics, and amino acids in sequence.

3 After the preparation of the cell culture medium is completed, it can be used to4°CStable storage in the refrigerator for up to4week

V2.0edition

Update time:2025/6/21