Mogengel® Cell culture plate (15 well)

NegotiableUpdate on 05/06

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Nature of the Manufacturer: Producers

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Place of Origin

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Overview

Mogengel Cell tube culture plate (15 well) is a high-quality consumable series product specifically designed for model based biological tube experiments, abbreviated as 15 well tube culture plate, suitable for forming tubular structures outside of cells.

Product Details

Mogengel^®Cell tube culture plate(15Kong)

♢The product is sterile, and the outer packaging of the product is non sterile. Please thoroughly disinfect the outer packaging of the product before use.

♢Once the product is opened, it should be stored properly to ensure its sterility.

♢The product is a disposable consumable for cell experiments. If it needs to be reused, please design your own operating procedures to ensure the sterility of reuse.

♢If only a portion of the holes are used during the experiment, alcohol cotton balls can be used to inactivate and wipe the holes after the experiment, and then sealed and stored for future use.

1 Product Description

Mogengel^®Cell tube culture plate(15Kong)It is the abbreviation for the series of high-quality consumables specifically designed for the tube forming experiment of modular biology15Kongcheng tube culture plate is suitable for forming tubular structures in vitro.

2 Product Information

Product Name	Product Item Number	specification	storage/transportation	shelf life
Mogengel^®Cell tube culture plate(15Kong)	MS-TBFMW15	1branch/package	Room temperature storage	60month

3 intended use

Mogengel^®Cell tube culture plate(15Kong)Used for endothelial cells or tumor cells in vitro3DenvironmentECM）A tubular cavity is formed in the middle to study the effects of certain genes or drugs on this process. Based on the theory that tumor growth and metastasis depend on angiogenesis, this experiment is also used to study the mechanism of tumor occurrence. This type of research is conducted in the process of tumor research, but the cost is expensive. This product aims to reduce the amount of reagents and cells used in tube experiments and lower the cost of scientific research.

Attention: Always wear when using, and follow safe laboratory procedures when handling substances such as human cells or other organisms and harmful substances.

Only for scientific research purposes.

4 Instructions for use: Organoid culture

a) Experimental supplies

Mogengel^®Cell tube culture plate(15Kong)TheMogengel^®MatrixStandard matrix adhesive (item number:0827045）Required cells and culture medium;

b) Experimental supplies

b.1, gel

Add to the lower chamber of the culture plate8-10MLMatrix adhesive, be careful not to produce bubbles. placed in37℃ gel in cell incubator5-15min.

b.2Cell experiments

After pre starvation or direct digestion of different cells to obtain the expected state of cells, resuspend and dilute them in cell culture medium to the corresponding number of cells, and extract them50MLjoinMogengel^®Cell tube culture plate(15Kong)In the middle, placed37Placement of ℃ incubator4-8hPre experiments can observe and confirm the experimental parameters of relevant cells at certain intervals.

watch1 HUVECCell usage for vascular formation experiment

Number of cells (pcs)	1*10⁴
The amount of cell culture medium used (μ g/mL)L）	50ML

b.3Cell experiments

Use an intelligent microscope system to capture the process of tracking tube formation, initiate real-time intelligent tube formation data analysis, and export the data. If there are no relevant devices, you can proceed to the steps according to the experimental requirements“b.4”.

b.4Cell experiments

1）Dye dilution: Use serum-free culture medium (such asDMEM）Diluted Calcein(12.5 µl calcein stock 1 µg/µl)The final concentration is6.25 µg/ml；

2）Remove the culture medium from the upper well and add10MlCalcein diluent;

3）Incubate at room temperature in the dark30After minutes, use sterilePBSWash three times;

4）Use485 nm/ 529 nmPerform immunofluorescence imaging on the channel.

b.5Image acquisition

After staining, use2The formation status of the magnifying glass collection tube (or directly collecting bright field images) is analyzed using image analysis software to analyze parameters such as tube area, tube length, tube quantity, ring area, ring quantity, and bud quantity for subsequent data analysis.