Shengxia Protein N-Glycoprotein Treatment Kit

NegotiableUpdate on 05/06

Model

Nature of the Manufacturer: Producers

Product Category

Place of Origin

Online Consult

Overview

The N-glycoprotein treatment reagents for Shengxia protein include BT surfactants, PNGase F glycosidase, and polysaccharide molecular labels. Used for rapid processing of antibody protein denaturation, deglycosylation, and derivatization of free sugars. The labeled N-glycans do not require HILIC column clean up treatment, and qualitative analysis and quantitative quality control are performed using chromatography and mass spectrometry. Fast processing of glycoprotein samples, short time, good reproducibility, low cost, and rapid mass spectrometry characterization of sugar types.

Product Details

Shengxia Protein N-Glycoprotein Treatment Kit

1.brand: Summer protein

application field：

biopharmaceuticalN-glycosylation analysis of therapeutic antibodies (such as monoclonal antibodies and bispecific antibodies).
quality controlCompliant with pharmacopoeia requirements (such as USP, EP), used for the consistency evaluation of sugar profiles between biosimilars and original drugs.
scientific researchCharacterization of glycoprotein structure and research on disease biomarkers.

2. Common reagent kit models and compositions

Our company provides various N-sugar analysis solutions, and typical reagent kits may include the following components:

Shengxia Protein N-Glycoprotein Treatment Kit(such as catalog number: HLS NGLY001)

RapiFlur MS labeling reagentFluorescent labeling (such as 2-AA, 2-AB analogues) enhances the sensitivity of mass spectrometry detection.
糖苷酶（PNGase F）Efficient release of N-sugar chains.
Buffer solution and purification columnUsed for the release, labeling, and purification of sugar chains.

3. Technical advantages

high sensitivityThe latest labeling technology increases sensitivity by 10-100 times compared to traditional labeling (such as 2-AB).
Quick processFrom sugar chain release to labeling, it only takes about 2 hours (traditional methods require overnight).
compatibility：

HPLC/UPLCAll are applicable.
mass spectrometry detectionSuitable for LC-MS combination (all products are mass spectrometry grade).

complianceSupport FDA/EMA requirements for biopharmaceutical sugar profile analysis.

4. Overview of Operation Process

Sugar chain releasePNGase F enzymatically hydrolyzes glycoproteins.
fluorescent labelingRapiFlur MS reagent reacts with sugar chains.
purificationUse matching columns to remove excess markers.
analysisUPLC separation (such as HILIC mode)+fluorescence/mass spectrometry detection.

5. Precautions for purchasing

Sample TypeConfirm if the reagent kit is suitable for your sample (such as monoclonal antibodies, serum glycoproteins).
Testing equipmentNeed to match various UPLCs or be compatible with other brands of HPLC (methods need to be optimized).
Flux demandEach kit contains 20 samples.