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22nd Floor, Guangqi City, No. 425 Yishan Road, Xuhui District, Shanghai
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Shanghai Boyi Biotechnology Co., Ltd
Reverse transcriptase packaging service
NegotiableUpdate on 05/06
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Overview
Retroviruses are derived from Moloney murine leukemia virus (Mo MLV), which can infect both human and mouse cells. The significant advantage of retroviral vectors is that they can convert single stranded RNA (ssRNA) genomes into ds DNA molecules for stable integration into the host genome. Retroviral vectors are suitable for dividing cells and other primitive cells. We can provide you with high-purity and high titer retroviruses.
Product Details
Service Content
Technical steps:
1. Cloning genes into expression vectors
2. Transfect retrovirus packaging cell lines, collect supernatant after cultivation to prepare virus crude extract
3. Virus purification and concentration
4. Determination of virus titer
5. Add virus solution to target cells and analyze the expression of recombinant target protein
Application areas: Stem cell transfection, RNAi， Genomics research, etc.
Service cycle: 2-3 weeks
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