Insect baculovirus is a universal expression system that involves the preparation of high titer and high-purity virus particles, as well as the production of large-scale recombinant proteins. The baculovirus expression system is highly popular due to its simple process, high expression level, and post-translational modification function.
Technical service content
1. Subcloning genes into an expression vector
2. Expansion culture of recombinant rod-shaped DNA for viral reproduction
3. Transfection of recombinant mitochondrial DNA into insect cells
4. Expand the culture of P1 virus storage solution (low titer concentration) and P2 virus storage solution (high titer concentration) to confirm the expression of virus titer protein.
5. Titer determination, PCR identification, and protein expression identification of insect rod-shaped viruses.
We can provide construction and preparation services for research grade insect baculoviruses for cell and animal experiments, with customers receiving titers of 10 within 2-3 months6-107Pfu/mL insect rod-shaped virus.