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    No. 88 Huangxi West Street, Lvxiang Town, Jinshan District, Shanghai

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Rabbit thioredoxin 1 (Trx-1) ELISA kit

NegotiableUpdate on 05/06
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Overview

The rabbit thioredoxin 1 (Trx-1) ELISA kit adopts a double antibody one-step sandwich enzyme-linked immunosorbent assay (ELISA), and the absorbance (OD value) is measured at 450nm wavelength by an enzyme-linked immunosorbent assay (ELISA) reader to calculate the sample concentration.

Product Details

Shanghai Xuanzekang Biotechnology Co., Ltd. provides free professional ELISA testing technology services (sample shipping costs are borne by customers), which can complete ELISA experiments in a short period of time, saving you time and money, and providing reliable experimental data. Scope of proxy testing:

1. Anyone who orders any ELISA test kit from our company will be provided with free testing services.

2. The service items include indirect ELISA detection, sandwich ELISA detection, and competitive ELISA detection.

3. Testing cycle: Within one week (5-7 working days) for spot products, the testing report (including sample raw data, testing sample correspondence table, standard curve analysis, sample data processing, and sample final results) will be handed over to the customer!

Rabbit thioredoxin 1 (Trx-1) ELISA kitPerformance:

1. Accuracy: The correlation coefficient R value between the standard linear regression and the expected concentration is greater than or equal to 0.9900.

2. Sensitivity: The detection concentration is less than 1.0 pg/mL.

3. Specificity: Does not cross react with other soluble structural analogues.

4. Repeatability: The coefficient of variation within and between plates is less than 15%.

5. Storage: Store at 2-8 ℃, away from light and moisture.

6. Validity period: 6 months

Rabbit thioredoxin 1 (Trx-1) ELISA kitComposition: (Please refer to the instruction manual for the detection range)

name

96 hole configuration

48 hole configuration

brand

Microporous enzyme-linked immunosorbent assay (ELISA) plate

12 holes x 8 strips

12 holes x 4 strips

Xuan Zekang

reference standard

0.3mL * 6 tubes

0.3mL * 6 tubes

Xuan Zekang

diluent

6mL

3mL

Xuan Zekang

Detect antibody HRP

10mL

5mL

Xuan Zekang

20 x washing buffer solution

25mL

15mL

Dilute according to the instructions

Substrate A

6mL

3mL

Xuan Zekang

Substrate B

6mL

3mL

Xuan Zekang

Stop Solution

6mL

3mL

Xuan Zekang

Sealing film

2 sheets

2 sheets

Xuan Zekang

Instruction manual

1 copy

1 copy

Xuan Zekang

self-sealing bag

1 piece

1 piece

Xuan Zekang

Security:

1. Avoid direct contact with colorants A, B, and termination solution by the human body. Once in contact with these liquids, please rinse with water as soon as possible.

Do not eat, smoke, or use cosmetics during the experiment.

3. Do not use your mouth to suck any ingredients from the reagent kit.

Precautions during operation:

1) After receiving the reagent kit, in order to ensure the detection effect, it is necessary to store it correctly according to the storage conditions in the instructions. Unless otherwise specified, most test kits are stored at 2-8 ℃.

2) Before conducting the experiment, please confirm that the components of the reagent kit in the packaging are consistent with the instructions before proceeding with subsequent operations.

3) In the process of dissolving the standard, pay attention to whether the standard is affected with moisture, and avoid foam in the process.

4) To avoid cross contamination, it is necessary to replace the nozzle when configuring different concentration standards, loading samples, and adding different reagents. Additionally, please use different pipettes for different reagents.

5) Proper use of sealing tape during each incubation can ensure the accuracy of the results.

6) Proper washing of the board helps to ensure the stability of experimental results.

7) The TMB chromogenic substrate should be colorless before being loaded onto the plate. Please store it away from light. After adding the orifice plate, it will change from colorless to varying degrees of blue.

8) The order of the termination solution on the plate should be consistent with the order of the substrate on the plate. Adding the termination solution will change the color of the solution from blue to yellow. If green appears, it indicates that the termination solution and substrate have not been mixed evenly. Please mix thoroughly.