Rabbit interleukin-1 (IL-1) ELISA kit for inflammatory cytokines

NegotiableUpdate on 05/06

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Nature of the Manufacturer: Producers

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Online Consult

Overview

The rabbit interleukin-1 (IL-1) ELISA kit was used to detect inflammatory factors using a double antibody one-step sandwich enzyme-linked immunosorbent assay (ELISA). The absorbance (OD value) was measured using an enzyme-linked immunosorbent assay (ELISA) reader at a wavelength of 450nm, and the sample concentration was calculated.

Product Details

Detection principle: The reagent kit adopts a double antibody one-step sandwich enzyme-linked immunosorbent assay (ELISA). Add the sample, standard, and HRP labeled detection antibody in sequence to the pre coated micropores with interleukin-1 antibody, incubate and wash. Using substrate TMB for color development, TMB is converted to blue under the catalysis of peroxidase and to the final yellow under the action of acid. The depth of color is positively correlated with the level of interleukin-1 (IL-1) in the sample. Measure the absorbance (OD value) at a wavelength of 450nm using an enzyme-linked immunosorbent assay (ELISA) reader and calculate the sample concentration.

Sample collection, processing, and preservation methods:Rabbit interleukin-1 (IL-1) ELISA kit for inflammatory cytokines

1. Serum: Use test tubes free of pyrogens and endotoxins, avoid any cell irritation during the operation, collect blood, centrifuge at 3000 rpm for 10 minutes, and quickly and carefully separate serum and red blood cells.

2. Plasma: anticoagulant with EDTA, citrate or heparin. Centrifuge at 3000 rpm for 30 minutes and collect the supernatant.

3. Cell supernatant: Centrifuge at 3000 rpm for 10 minutes to remove particles and polymers.

4. Tissue homogenate: Crush the tissue by adding an appropriate amount of physiological saline. Centrifuge at 3000 rpm for 10 minutes and collect the supernatant.

5. Storage: If the sample is not tested in a timely manner after collection, please divide it into batches according to a single dose, freeze it at -20 ℃, avoid repeated freezing and thawing, thaw it at room temperature, and ensure that the sample is evenly filled and thawed.

Rabbit interleukin-1 (IL-1) ELISA kit for inflammatory cytokinesComposition: (Please refer to the instruction manual for the detection range)

name	96 hole configuration	48 hole configuration	brand
Microporous enzyme-linked immunosorbent assay (ELISA) plate	12 holes x 8 strips	12 holes x 4 strips	Xuan Zekang
reference standard	0.3mL * 6 tubes	0.3mL * 6 tubes	Xuan Zekang
diluent	6mL	3mL	Xuan Zekang
Detect antibody HRP	10mL	5mL	Xuan Zekang
20 x washing buffer solution	25mL	15mL	Dilute according to the instructions
Substrate A	6mL	3mL	Xuan Zekang
Substrate B	6mL	3mL	Xuan Zekang
Stop Solution	6mL	3mL	Xuan Zekang
Sealing film	2 sheets	2 sheets	Xuan Zekang
Instruction manual	1 copy	1 copy	Xuan Zekang
self-sealing bag	1 piece	1 piece	Xuan Zekang

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