Quantitative detection of bovine triglycerides (TG) ELISA kit

Preparation points before the experiment:

1. Prepare various experimental instruments and materials (such as a 37 ℃ constant temperature incubator, 450nm enzyme-linked immunosorbent assay reader, high-speed centrifuge, high-precision sampler and gun head, etc.)

2. According to the safety protection regulations of the biological laboratory, wear laboratory clothes and latex gloves to do a good job of protection

Before use, all reagents in the kit should be removed and equilibrated to room temperature (at least 30 minutes)

4. Strictly follow the operating instructions in the manual. The instruction manual shall be based on the English version.

Quantitative detection of bovine triglycerides (TG) ELISA kitcomposition

【 Enzyme linked Assay Plate 】, 【 Standard 】, 【 Diluent Sample 】, 【 HRP Conjugate reagent 】, 【 Stop Solution 】, 【 Chromogen Solution 】, 【 20x Concentrated Wash Solution 】, 【 User Manual 】, 【 Closure Plate Membrane 】, 【 Self sealing Sealed Bags 】

Quantitative detection of bovine triglycerides (TG) ELISA kitFrequently Asked Questions:

1. Question: What is the sample size for the reagent kit?

Shanghai Xuanzekang Biotechnology Answer: Add 10ul of sample. First add 40ul of diluent, then add 10ul of sample.

2. Question: Can the detection range of the reagent kit be adjusted?

Shanghai Xuanzekang Biotechnology Answer: It can be adjusted. Different manufacturers have different testing ranges due to the use of different standard products. The detection range is set based on the actual content of different samples, and this detection range should try to meet relatively low conditions, such as serum plasma, tissue homogenate, cell samples, etc.

3. Question: What should I pay attention to when sending samples for free testing?

Shanghai Xuanzekang Biotechnology Answer: Anyone who purchases our ELISA kit can be tested for free. Requirements for sending samples: samples are packed in EP tubes/centrifuge tubes/cryopreservation tubes, marked with numbers, transported in dry ice+foam boxes, and SF Express is preferred.

4. Question: How many samples need to be sent?

Shanghai Xuanzekang Biotechnology Answer: Serum and other liquid phase samples depend on the number of surrogate indicators and whether repeated testing is performed. For example, for testing three indicators, each sample needs to be tested twice, and each sample experiment requires 60ul. To prevent loss during transportation and the experimental process, an additional 5-10ul can be sent. Fresh tissue samples need to be sent in a quantity of no less than 50mg.

5. Question: Can the reagent kit be used in batches?

Shanghai Xuanzekang Biotechnology Answer: Our enzyme-linked immunosorbent assay (ELISA) plates can be disassembled and used. Unused reagents and reagent kits should be sealed and stored in a refrigerator at 2-8 ℃. Valid for 6 months.

We provide technical guidance throughout the experiment (from pre-sales specimen collection to data analysis after the experiment), and Shanghai Xuanzekang Biotechnology guarantees return and exchange for any quality issues with the reagent kit. At the same time, our company promises to provide free testing services for any ELISA test kit ordered, saving you time. You only need to send the specimen to our company, and we can provide the experimental results, raw data, and analysis data.

Our commonly used testing indicators are:

1. Cytokine detection (such as interleukin, necrosis factor, interferon, transforming growth factor, chemokine, cytokine receptor, colony-stimulating factor, adhesion molecule, growth factor, etc.)

2. Endocrine testing (such as thyroid, pancreas, sex, progesterone, growth, endothelin, osteocalcin, prolactin, testosterone, adrenocorticotropic hormone, follicle stimulating hormone, estradiol, estriol, serotonin, 17 hydroxyprogesterone, etc.)

3. Myocardial infarction testing (such as troponin, myoglobin, C-reactive protein, etc.)

4. Liver fibrosis detection (such as fibronectin, hyaluronic acid, collagen, matrix metalloproteinase factor, matrix metalloproteinase, laminin, etc.)