Rabbit osteoprotegerin (OPG) enzyme-linked immunosorbent assay (ELISA) kit

Rabbit osteoprotegerin (OPG) enzyme-linked immunosorbent assay (ELISA) kitPrecautions before the experiment:

Before the experiment begins, arrange the experimental process and clean the workbench.

2. Check for unstable or deteriorated reagent solutions (such as precipitation or discoloration), and do not use reagents from different production batches to replace or mix existing reagents.

3. Avoid foam when mixing or dissolving protein solution.

4. Our company is only responsible for the reagent kit itself and is not responsible for the sample consumption caused by the use of the reagent kit. Please fully consider the required sample size before conducting the experiment.

5. Conduct the experiment strictly according to the instructions, and any step is indispensable (as it will lead to the failure of the experiment)

Rabbit osteoprotegerin (OPG) enzyme-linked immunosorbent assay (ELISA) kitComposition: (Please refer to the instruction manual for the detection range)

Preparation of experimental materials and reagents:

1. Instruments and materials: ELISA reader (preheated for 30 minutes before use), micropipette, pipette, distilled water or deionized water, filter paper.

2. Dilution of 20 x washing buffer: Dilute distilled water at a ratio of 1:20, which means adding 19 parts of distilled water to 1 part of 20 x washing buffer.

Sample collection, processing, and preservation:

Cell culture supernatant: suitable for detecting secreted components of cells cultured in vitro. Collect the cell supernatant using a sterile tube, centrifuge at 1000 × g for 15 minutes, and collect the supernatant.

Serum: At room temperature, blood naturally coagulates, centrifuge at 1000 × g for 15 minutes, and collect the supernatant for testing.

【 Plasma 】: EDTA or sodium citrate should be selected as anticoagulants according to the requirements of the specimen. After mixing, let it stand for 10-20 minutes, centrifuge at 1000 × g for 15 minutes, and collect the supernatant.

【 Body fluids 】: including pleural and peritoneal fluid, cerebrospinal fluid, secretions, etc. Collect using a centrifuge tube without pyrogen and endotoxin, centrifuge at 1000 × g for 15 minutes, and collect the supernatant.

: The sample cannot contain NaN3, as NaN3 inhibits the activity of horseradish peroxidase (HRP).

: If the sample cannot be immediately detected, it should be packaged and stored at -80 ℃ to avoid repeated freezing. If precipitation occurs during storage, it should be centrifuged again. Do not use hemolytic or hyperlipidemic blood samples as much as possible. If there are a large number of particles in the serum, centrifuge or filter it before testing. Do not thaw by heating at 37 ℃ or higher. Thaw at room temperature and ensure that the sample is evenly thawed.