Rabbit Matrix Metalloproteinase 1 (MMP-1) Scientific ELISA Kit

:Rabbit Matrix Metalloproteinase 1 (MMP-1) Scientific ELISA Kit

: In stock supply

[Brand]: Xuanzekang Biotechnology

: 96T/box: can detect 90 samples; 48T/box: can detect 42 samples

[Purpose of Detection]: Used for quantitative detection of human XX content in serum, plasma, tissue homogenate, and related liquid samples in vitro.

Sensitivity: 1.0 ng/mL

[Specificity]: Does not cross react with other soluble structural analogues.

[Repeatability]: The coefficient of variation within and between plates is less than 15%.

【 Complete Manual 】: The manual is sent with the goods, or you can directly contact customer service personnel to obtain the electronic version.

Sample collection, processing, and preservation methods:

1. Serum: Use test tubes free of pyrogens and endotoxins, avoid any cell irritation during the operation, collect blood, centrifuge at 3000 rpm for 10 minutes, and quickly and carefully separate serum and red blood cells.

2. Plasma: anticoagulant with EDTA, citrate or heparin. Centrifuge at 3000 rpm for 30 minutes and collect the supernatant.

3. Cell supernatant: Centrifuge at 3000 rpm for 10 minutes to remove particles and polymers.

4. Tissue homogenate: Crush the tissue by adding an appropriate amount of physiological saline. Centrifuge at 3000 rpm for 10 minutes and collect the supernatant.

5. Storage: If the sample is not tested in a timely manner after collection, please divide it into batches according to a single dose, freeze it at -20 ℃, avoid repeated freezing and thawing, thaw it at room temperature, and ensure that the sample is evenly filled and thawed.

Self provided items:

1. ELISA reader (450nm)

2. High precision sampler and nozzle: 0.5-10uL, 2-20uL, 20-200uL, 200-1000uL

3. 37 ℃ constant temperature box

Rabbit Matrix Metalloproteinase 1 (MMP-1) Scientific ELISA KitNotes:

1. Disposable suction tips must be used during experimental operations to avoid cross contamination.

2. Sample addition: When adding samples, the sample addition speed should be controlled to avoid excessive time intervals between wells, otherwise it will result in different pre incubation times, which will affect the accuracy and repeatability of the experiment.

3. Incubation: Strictly follow the incubation time and temperature specified in the instructions.

4. Control of reaction time: After adding the substrate, please observe the color change of the reaction well regularly. If the color is darker, please add a stop solution in advance to terminate the reaction.

5. It is recommended to predict the sample content before the experiment. If the sample concentration is too high, the sample should be diluted and the calculation result should be multiplied by the corresponding dilution factor.

6. It is recommended to conduct a pre experiment (i.e., test a few specimens with a standard curve) before using this kit. If you have any questions about this kit, you can contact the purchased distributor. If the kit fails due to transportation, you can request replacement, but we will not be responsible for any losses outside of the product itself.