Primary cells of human small airway epithelium

All products of our company are for scientific experiments only and are not intended for use outside of scientific experiments!

Product Name:Primary cells of human small airway epithelium
English name:Human Small Airway Epithelial Cells
Specifications5×10⁵Cells/T25culture flask
Item No.GOY-01X0725
Classification:Human primary cells

Growth characteristics: Wall adhesion

Cell morphology:Epithelial like cells

Isolation of human small airway epithelial cells from small airways; In clinical practice, the inner diameter is usually smaller than2mmThe small bronchioles are called small airways. The stingy prop has the characteristic of low airflow resistance but easy blockage. When inhaling calmly, air enters the narrow nasopharynx, creating vortices. Due to the lack of cartilage support in the small airway, after detaching from the fibrous sheath and embedding into the lung tissue, the patency of the lumen is not as good as that of the cartilaginous airway, and is easily affected by changes in pressure in the chest cavity. The epithelial cells of the small airways form a continuous inner layer of the respiratory tract, playing a role as a physical and functional barrier to isolate harmful substances from the outside world. The small airways are located at the junction of the alveoli and trachea, and these cells are capable of regulating immune responses, producing chemical factors for host defense, expressing adhesion molecules, and possibly throughHLA-DRExpressing and presenting antigens; They can also produce fluids that contribute to the balance of lung fluid. Many respiratory diseases, such as asthma, bronchitis, chronic obstructive pulmonary disease, and cystic fibrosis, involve the destruction of surface epithelial cells in the respiratory tract; The cultivation of small airway epithelial cells can provide a new therapeutic option for preventing respiratory expansion diseases and remodeling. The physiological characteristics of small airways: ① Small airway resistance; ② Slow airflow speed; ③ Adjustable control of ventilation and blood flow ratio.

Method Introduction:

The human small airway epithelial cells isolated in the company laboratory were treated with protease-The collagenase mixed digestion method combined with differential adhesion method was prepared by culturing and screening with epithelial cell specific culture medium, with a total cell count of approximately5×10?cells/Bottle.

Quality inspection:

The human small airway epithelial cells isolated from the company laboratoryPCKImmunofluorescence identification, purity can reach90%Above, and not containingHIV-1TheHBVTheHCVMycoplasma, bacteria, yeast, fungi, etc.

1. Preparation of culture medium and culture cryopreservation conditions:

Prepare DMEM high sugar, 10% high-quality fetal bovine serum.

1) Cultivation conditions: Gas phase: air, 95%; Carbon dioxide, 5%. Temperature: 37 degrees Celsius, humidity in the incubator is 70% -80%.

2) Cryogenic solution: 90% culture medium, 10% DMSO, ready to use and prepared. Liquid nitrogen storage.

2. Cell processing:

1) Resuscitate cells: Quickly shake and thaw a cryovial containing 1mL of cell suspension in a 37 ℃ water bath, then add 4mL of culture medium and mix well. Centrifuge at 1000RPM for 4 minutes, discard the supernatant, add 1-2mL of culture medium, and blow evenly. Then add all cell suspensions to the culture bottle and culture overnight (or add the cell suspensions to a 10cm dish, add about 8ml of culture medium, and culture overnight). The next day, change the solution and check the cell density.

2) Cell passage: If the cell density reaches 80% -90%, passage culture can be carried out.

3) Cell cryopreservation: When the cells are in good growth condition, cell cryopreservation can be performed. When freezing adherent cells, discard the culture medium and add a small amount. After the cells become round and fall off, add about 1ml of serum containing culture medium to the freezing tube, and then add 10% DMSO for freezing.

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1. After receiving the cells, first observe whether the cell bottle is intact and whether there is any leakage or turbidity in the culture medium. If any of the above phenomena occur, please contact us in a timely manner.

2. Carefully read the cell manual to understand cell related information, such as cell morphology, culture medium used, serum ratio, required cytokines, etc.

3. Wipe the surface of the cell vial with 75% alcohol and observe the cell state under a microscope. Due to transportation issues, a small amount of adherent cells may detach from the bottle wall. The cells should be placed in a culture box and incubated overnight before being taken out for observation the next day. At this point, most cells will adhere to the wall. If the cells still cannot adhere to the wall, please use trypan blue staining to determine cell viability. If it is confirmed that the cell viability is normal, please centrifuge the cells and culture them again with fresh culture medium; If the staining result shows that the cells are inactive, please take a photo and contact us in a timely manner. After confirming the information, we will send it to you again for free.

4. Please ask the customer to use the same culture medium for cell culture under the same conditions. Excess culture medium in the culture bottle can be collected for future use. When cells are passaged, they can be mixed in a certain proportion with the culture medium provided by the customer to gradually adapt to the culture conditions; Suggest purchasing the provided culture medium directly.

5. It is recommended that customers take several photos of the cells in the first 3 days after receiving them, recording the cell status for communication and exchange with the technical department of the Microbial Strain Query Network.

6. This cell can only be used for scientific research and cannot be used for clinical applications.