Primary cells of human dermal lymphoid epithelium

Primary cells of human dermal lymphoid epithelium

Training Information:

Coated condition: Mouse tail collagen I (2-5 μ g/cm2)

The culture medium contains FBS, growth additives, Penicillin, Streptomycin, etc

Change fluid frequency every 2-3 days

Growth characteristics: Wall adhesion

Cell morphology epithelial cell like

Passage characteristics can be passed down for 2-3 generations

Digestive fluid 0.25%

Cultivation conditions: Air, 95%; CO2，5%

Isolation of human dermal lymphoid epithelial cells from dermal tissue; The dermis is located deep in the epidermis and is connected to subcutaneous tissue downwards. The collagen fibers and elastic fibers of the dermal connective tissue are interwoven and buried in the matrix. It is distributed with various connective tissue cells and a large number of collagen fibers and elastic fibers, making the skin both elastic and resilient. It consists of two layers: the nipple layer and the mesh layer. The structural composition of genuine leather is collagen, elastic fibers, and matrix. Microvascular endothelial cells (MECs) play a crucial role in inflammatory responses, tumor growth, and wound healing processes. In the field of wound healing research, due to the maturity of in vitro culture techniques for epidermal cells and dermal fibroblasts, extensive and in-depth research has been conducted on these two types of cells.

Method Introduction:

The human dermal lymphoid epithelial cells isolated in the company's laboratory were prepared using a protease collagenase mixed digestion method combined with differential adhesion method, and were cultured and screened using a specialized epithelial cell culture medium. The total number of cells was approximately 5 × 10? Cells/bottle.

Quality inspection:

The human dermal lymphoid epithelial cells isolated from the company's laboratory were identified by PCK immunofluorescence, with a purity of over 90%, and did not contain HIV-1, HBV, HCV, mycoplasma, bacteria, yeast, fungi, etc.

① Organizational block cultivation method

Tissue block culture is a commonly used, simple, and highly successful primary culture method. The basic method is to inoculate the cut small tissue clusters into a culture bottle (or dish), and the bottle wall can be pre coated with a thin layer of collagen to facilitate the adhesion of the tissue blocks to the bottle wall, allowing surrounding cells to grow outward along the bottle wall.

② Digestive cultivation method

③ Suspension cell culture method

For cells that grow in suspension, such as leukemia cells, lymphocytes, bone marrow cells, cancer cells and immune cells in pleural and ascites, digestion is not required. They can be isolated by low-speed centrifugation and cultured directly, or cultured by inoculating after lymphocyte stratification.

④ Organ culture

Organ culture refers to the direct cultivation of organs or tissue blocks obtained from donors under specific environmental conditions outside the body without tissue separation. Organ culture can maintain the relative integrity of organ tissues and can be used to focus on observing the connections, arrangements, and interactions between cells, as well as the biological regulatory effects of local environments.

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Recombinant Prostaglandin E Synthase (PTGES)

JAG1 Protein HumanRecombinant human JAG1/Jagged 1/CD339 protein (Fc tag)

SELERecombinant human E-Selectin/CD62e/SELE protein (His&Fc tag) protein

HA Protein H9N2Recombinant influenza A H9N2 (A/Chicken/Hong Kong/G9/97) hemagglutinin HA1 protein

ACOT13Recombinant human THEM2/ACOT13 protein

Rat tissue transglutaminase (TGM2) kit, English name: TGM2 ELISA Kit

Mouse ai alpha fodrin aibody IgG/IgA (-Fodrin IgG/IgA) ELISA KitMouse anti alpha cytoskeletal protein antibody IgG/IgA kit

MousehepatitisBviruseaigen,HBeAgELISAKitMouse hepatitis B e antigen (HBeAg) kit 96T/48T imported packaging

CLIAKitforHumanmembranecofactorprotein,MCPELISAKitHuman membrane co protein

Transmission Electron Microscopy (TEM) Universal Mesh Tissue Sample Negative Staining Kit 20 times

ELISAKitsP-selectinSoluble P-selectin in rats

Rabbit platelet activating factor (rabbit PAF) function: ELISA specification: imported packaged

Rabbit P-selectin Function: ELISA Specification: Imported Packaging

Rabbit platelet-derived growth factor (rabbit PDGF) function: ELISA specification: imported packaging

Rabbit Raes Effect: ELISA Specification: Imported Packaging

Primary cells of human dermal lymphoid epitheliumMouse (VD) ELISA Kit 96T/48T

Rat bone morphogenetic protein (BMPs) ELISA KitRat Bone Forming Proteins (BMPs) Kit

HumanProteindisulfide-isomeraseprecursor,PDIELISAKitHuman Protein Disulfide Isomerase Precursor (PDI) Kit 96T/48T Imported Packaging

ChickenLaminin,LNChicken Laminin/Laminin (LN) Kit Specification: 96T/48T

Fluorescence microscopy kit for IP3R receptor protein expression in glass slide cells 10/20 times

MouseGelsolinELISAKitMouse Gelsolin Kit Specification: 96T/48T

PDCD1Recombinant dog PD1/PDCD1/CD279 protein

Natrexone/BSA:Natrexone/KLHNatrexone conjugated bovine serum albumin: Natrexone conjugated hemocyanin 0.5mg/BSA: Natrexone/KLH Natrexone conjugated bovine serum albumin: Natrexone conjugated hemocyanin

SIRPGRecombinant human SIRPG/SIRP gamma/CD172g protein

CREB3L1 Protein HumanRecombinant human CREB3L1/OASIS protein (aa 396-519, His tag)

NTRK1 Protein MouseRecombinant mouse TrkA/NTRK1 protein (Fc tag)

After receiving the cells, please proceed with the following steps for the customer. (The number of passages for this cell is limited to about 2. It is recommended that the customer arrange subsequent experiments as soon as they receive the cells.)

1. Remove the cell vial, disinfect it with 75% alcohol, remove the sealing film, and place it in a 37 ℃, 5% CO2 incubator for 6-8 hours to stabilize the cell state.

2. When the cells reach 80% confluence, prepare for subculture.

3. Cell passage:

1) Suck out the culture medium from the cell vial and wash the cells once with PBS.

2) Add about 1mL of 0.125% digestive solution to the culture bottle and digest at 37 ℃ for about 3 minutes; Under the microscope, after the cells shrink and become round, discard the digestion solution and add culture medium to terminate digestion.

3) Gently blow and mix with a straw, inoculate and passage in appropriate ratios such as 1:2 or 1:3, then add fresh culture medium to 5mL and culture in a 37 ℃ 5% CO2 cell incubator.

4) After the cells adhere to the wall, culture and observe, and replace with fresh culture medium every 2-3 days.